Lasers in Medical Science

, Volume 29, Issue 2, pp 757–763 | Cite as

Protective effect of low-level laser therapy (LLLT) on acute zymosan-induced arthritis

  • Fernando Pereira Carlos
  • Marcelo de Paula Alves da Silva
  • Eliadna de Lemos Vasconcelos Silva Melo
  • Maricilia Silva Costa
  • Stella Regina ZamunerEmail author
Original Article


The aim of this study was to evaluate the effect of low-level laser therapy on acute zymosan-induced arthritis, with respect to the laser action on inflammatory cells influx, release of pro-inflammatory mediators, metalloproteinases activity into the joint cavity and the cartilage repair process. Arthritis was induced in male Wistar rats (250–280 g) by intra-articular injection of zymosan (1 mg dissolved in 50 μl of a sterile saline solution) into one rear knee joint. Animals were irradiated immediately, 1 and 2 h after zymosan administration with a semiconductor laser InGaAIP (660 nm, 10 mW, 2.5 J/cm2, 10 s). In the positive control group, animals were injected with the anti-inflammatory drug dexamethasone 1 h prior to the zymosan administration. Treatment with laser significantly inhibited leukocytes influx, the release of IL-1 and IL-6 and also the activity of metalloproteinase-2 and 9, into the joint cavity. In conclusion, laser therapy was effective in reducing inflammation to sites of injury and inhibit activation of proteases (gelatinase) suggesting less degradation of collagen tissue in experimental model of acute arthritis.


Inflammation Arthritis LLLT Cytokines MMP 



The authors would like to acknowledge financial support from the Brazilian Council of Research—CNPq (Process no. 475083/2011-3).


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Copyright information

© Springer-Verlag London 2013

Authors and Affiliations

  • Fernando Pereira Carlos
    • 1
  • Marcelo de Paula Alves da Silva
    • 1
  • Eliadna de Lemos Vasconcelos Silva Melo
    • 1
  • Maricilia Silva Costa
    • 2
  • Stella Regina Zamuner
    • 1
    • 3
    Email author
  1. 1.Universidade Nove de JulhoSão PauloBrazil
  2. 2.Institute of Research and Development, University of Vale do ParaíbaSão José dos CamposBrazil
  3. 3.São PauloBrazil

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