Lasers in Medical Science

, Volume 29, Issue 1, pp 121–129 | Cite as

The effects of low-level laser irradiation on cellular viability and proliferation of human skin fibroblasts cultured in high glucose mediums

  • Mohammad Esmaeelinejad
  • Mohammad BayatEmail author
  • Hasan Darbandi
  • Mehrnoush Bayat
  • Nariman Mosaffa
Original Article


Delayed wound healing is one of the most challenging complications of diabetes mellitus (DM) in clinical medicine. This study has aimed to evaluate the effects of low-level laser therapy (LLLT) on human skin fibroblasts (HSFs) cultured in a high glucose concentration. HSFs were cultured either in a concentration of physiologic glucose (5.5 mM/l) or high glucose media (11.1 and15 mM/l) for either 1 or 2 weeks after which they were subsequently cultured in either the physiologic glucose or high concentration glucose media during laser irradiation. LLLT was carried out with a helium–neon (He–Ne) laser unit at energy densities of 0.5, 1, and 2 J/cm2, and power density of 0.66 mW/cm2 on 3 consecutive days. HSFs’ viability and proliferation rate were evaluated with the dimethylthiazol-diphenyltetrazolium bromide (MTT) assay. The LLLT at densities of 0.5 and 1 J/cm2 had stimulatory effects on the viability and proliferation rate of HSFs cultured in physiologic glucose (5.5 mM/l) medium compared to their control cultures (p = 0.002 and p = 0.046, respectively). All three doses of 0.5, 1, and 2 J/cm2 had stimulatory effects on the proliferation rate of HSFs cultured in high glucose concentrations when compared to their control cultures (p = 0.042, p = 0.000, and p = 0.000, respectively). This study showed that HSFs originally cultured for 2 weeks in high glucose concentration followed by culture in physiologic glucose during laser irradiation showed enhanced cell viability and proliferation. Thus, LLLT had a stimulatory effect on these HSFs.


Helium–neon laser Diabetes mellitus Human skin fibroblasts MTT assay Proliferation rate Viability 



We thank the late Mrs. Jamileh Rezaei. We also extend our thanks to the Vice Chancellor of Research at Shahid Beheshti University of Medical Sciences, Tehran, Iran, for financial support.

Conflict of interest

No competing financial interests exist.


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Copyright information

© Springer-Verlag London 2013

Authors and Affiliations

  • Mohammad Esmaeelinejad
    • 1
  • Mohammad Bayat
    • 2
    Email author
  • Hasan Darbandi
    • 3
  • Mehrnoush Bayat
    • 4
  • Nariman Mosaffa
    • 3
  1. 1.Dental FacultyShahid Beheshti University of Medical SciencesTehranIran
  2. 2.Cellular and Molecular Biology Research CenterShahid Beheshti University of Medical SciencesTehranIran
  3. 3.Department of Immunology, Medical FacultyShahid Beheshti University of Medical SciencesTehranIran
  4. 4.Dental FacultyTehran University of Medical SciencesTehranIran

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