Lasers in Medical Science

, Volume 29, Issue 1, pp 97–104 | Cite as

Effect of 830-nm diode laser irradiation on human sperm motility

  • Reza Salman Yazdi
  • Simin Bakhshi
  • Firooz Jannat Alipoor
  • Mohammad Reza Akhoond
  • Soheila Borhani
  • Faramarz Farrahi
  • Mehdi Lotfi Panah
  • Mohammad Ali Sadighi Gilani
Original Article


Sperm motility is known as an effective parameter in male fertility, and it depends on energy consumption. Low-level laser irradiation could increase energy supply to the cell by producing adenosine triphosphate. The purpose of this study is to evaluate how the low-level laser irradiation affects the human sperm motility. Fresh human semen specimens of asthenospermic patients were divided into four equal portions and irradiated by 830-nm GaAlAs laser irradiation with varying doses as: 0 (control), 4, 6 and 10 J/cm2. At the times of 0, 30, 45 and 60 min following irradiation, sperm motilities are assessed by means of computer-aided sperm analysis in all samples. Two additional tests [HOS and sperm chromatin dispersion (SCD) tests] were also performed on the control and high irradiated groups as well. Sperm motility of the control groups significantly decreased after 30, 45 and 60 min of irradiation, while those of irradiated groups remained constant or slightly increased by passing of time. Significant increases have been observed in doses of 4 and 6 J/cm2 at the times of 60 and 45 min, respectively. SCD test also revealed a non-significant difference. Our results showed that irradiating human sperms with low-level 830-nm diode laser can improve their progressive motility depending on both laser density and post-exposure time.


Sperm motility Low-level laser HOS test SCD test 



We would like to thank the all staffs and patients of Royan Institute Clinical Laboratory for providing us the semen samples and analysing facilities, especially laboratory technicians Abdol-Ali Ansary and Kaveh Afraz.


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Copyright information

© Springer-Verlag London 2013

Authors and Affiliations

  • Reza Salman Yazdi
    • 1
    • 5
  • Simin Bakhshi
    • 2
  • Firooz Jannat Alipoor
    • 1
  • Mohammad Reza Akhoond
    • 3
  • Soheila Borhani
    • 4
  • Faramarz Farrahi
    • 1
  • Mehdi Lotfi Panah
    • 3
  • Mohammad Ali Sadighi Gilani
    • 1
  1. 1.Department of Andrology at Reproductive Biomedicine Research CenterRoyan Institute for Reproductive Biomedicine, ACECRTehranIran
  2. 2.LASER DepartmentGolha ClinicTehranIran
  3. 3.Department of Epidemiology and Reproductive Health at Reproductive Epidemiology Research CenterRoyan Institute for Reproductive Biomedicine, ACECRTehranIran
  4. 4.Shahid Beheshti University of Medical ScienceTehranIran
  5. 5.Royan InstituteTehranIran

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