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Lasers in Medical Science

, Volume 28, Issue 6, pp 1467–1474 | Cite as

Phototherapy and resistance training prevent sarcopenia in ovariectomized rats

  • Adalberto Vieira CorazzaEmail author
  • Fernanda Rossi Paolillo
  • Francisco Carlos Groppo
  • Vanderlei Salvador Bagnato
  • Paulo Henrique Ferreira Caria
Original Article

Abstract

The aim of this study was to histologically and biochemically analyze the effects of light-emitting diode therapy (LEDT) associated with resistance training to prevent sarcopenia in ovariectomized rats. Forty female Wistar rats (12 months old, 295–330 g) were bilaterally ovariectomized and divided into four groups (n = 10 per group): control–sedentary (C), resistance training (T), LEDT–sedentary (L), and LEDT plus resistance training (LT). Trained rats performed a 12-week water-jumping program (3 days per week) carrying a load equivalent to 50–80 % of their body mass strapped to their back. Depending on the group protocol, the LED device (850 nm, 100 mW, 120 J/cm2, spot size 0.5 cm2) was used either as the only method or after the resistance training had been performed. The device was used in the single point contact mode (for 10 min). The irradiated region was the center of the greater trochanter of the right femur and the middle third of the rectus femoris muscle was subsequently analyzed histomorphometrically. Significant increases (p < 0.05) were noted for the muscle volume of the T (68.1 ± 19.7 %), the L (74.1 ± 5.1 %), and the LT (68.2 ± 11.5 %) groups compared to the C group (60.4 ± 5.5 %). There were also significant increases in the concentrations of IGF-1, IL-1, and TNF-α in the muscles of the treated groups (p < 0.05). Animals in the LT group showed a significant increase in IL-6 compared to T, L, and C groups (p < 0.05). These findings suggest that resistance training and LEDT can prevent sarcopenia in ovariectomized rats.

Keywords

LEDT Ovariectomy Phototherapy Resistance training Sarcopenia 

Notes

Acknowledgments

The authors would like to thank Felippe Furlan, Júlio César Domingues, Luis Vaz de Lima Júnior, and Mariana Sabino Bortolozzo for valuable technical assistance. We would also like to thank the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for their financial support.

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Copyright information

© Springer-Verlag London 2013

Authors and Affiliations

  • Adalberto Vieira Corazza
    • 1
    Email author
  • Fernanda Rossi Paolillo
    • 2
  • Francisco Carlos Groppo
    • 1
  • Vanderlei Salvador Bagnato
    • 2
  • Paulo Henrique Ferreira Caria
    • 1
  1. 1.Department of Morphology, Piracicaba Dental School (FOP)University of Campinas (UNICAMP)PiracicabaBrazil
  2. 2.Optics Group from Physics Institute of São Carlos (IFSC)University of São Paulo (USP)São CarlosBrazil

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