Lasers in Medical Science

, Volume 28, Issue 4, pp 1113–1117 | Cite as

Low-level visible light (LLVL) irradiation promotes proliferation of mesenchymal stem cells

  • Anat Lipovsky
  • Uri Oron
  • Aharon Gedanken
  • Rachel LubartEmail author
Original Article


Low-level visible light irradiation was found to stimulate proliferation potential of various types of cells in vitro. Stem cells in general are of significance for implantation in regenerative medicine. The aim of the present study was to investigate the effect of low-level light irradiation on the proliferation of mesenchymal stem cells (MSCs). MSCs were isolated from the bone marrow, and light irradiation was applied at energy densities of 2.4, 4.8, and 7.2 J/cm2. Illumination of the MSCs resulted in almost twofold increase in cell number as compared to controls. Elevated reactive oxygen species and nitric oxide production was also observed in MSCs cultures following illumination with broadband visible light. The present study clearly demonstrates the ability of broadband visible light illumination to promote proliferation of MSCs in vitro. These results may have an important impact on wound healing.


Mesenchymal stem cells Proliferation Visible light 



The authors wish to thank Dr. Tuby H. and Maltz L. for their help with the isolation of MSC.

Conflict of interest

All authors have completed and submitted the ICMJE Form for Disclosure of Potential Conflicts of Interest and none were reported.


  1. 1.
    Baffour R, Pakala R, Hellinga D, Joner M, Okubagzi P, Epstein SE, Waksman R (2006) Bone marrow-derived stem cell interactions with adult cardiomyocytes and skeletal myoblasts in vitro. Cardiovasc Revasc Med 7:222–230PubMedCrossRefGoogle Scholar
  2. 2.
    Broughton G 2nd, Janis JE, Attinger CE (2006) The basic science of wound healing. Plast Reconstr Surg 117:12S–34SPubMedCrossRefGoogle Scholar
  3. 3.
    Robson MC (1991) Growth factors as wound healing agents. Curr Opin Biotechnol 2:863–867PubMedCrossRefGoogle Scholar
  4. 4.
    Jeon YK, Jang YH, Yoo DR, Kim SN, Lee SK, Nam MJ (2010) Mesenchymal stem cells’ interaction with skin: wound-healing effect on fibroblast cells and skin tissue. Wound Repair Regen 18:655–661PubMedCrossRefGoogle Scholar
  5. 5.
    Richardson GD, Arnott E, Whitehouse CJ, Lawrence CM, Hole N, Jahoda CAB (2005) Cultured cells from the adult human hair follicle dermis can be directed toward adipogenic and osteogenic differentiation. J Invest Dermatol 124:1090–1091PubMedCrossRefGoogle Scholar
  6. 6.
    Hanson SE, Bentz ML, Hematti P (2010) Mesenchymal stem cell therapy for nonhealing cutaneous wounds. Plast Reconstr Surg 125:510–516PubMedCrossRefGoogle Scholar
  7. 7.
    Rochefort GY, Delorme B, Lopez A, Herault O, Bonnet P, Charbord P, Eder V, Domenech J (2006) Multipotential mesenchymal stem cells are mobilized into peripheral blood by hypoxia. Stem Cells 24:2202–2208PubMedCrossRefGoogle Scholar
  8. 8.
    Lubart R, Lavi R, Friedmann H, Rochkind S (2006) Photochemistry and photobiology of light absorption by living cells. Photomed Laser Surg 24:179–185PubMedCrossRefGoogle Scholar
  9. 9.
    Gao X, Xing D (2009) Molecular mechanisms of cell proliferation induced by low power laser irradiation. J Biomed Sci 16:4PubMedCrossRefGoogle Scholar
  10. 10.
    Demidova-Rice TN, Salomatina EV, Yaroslavsky AN, Herman IM, Hamblin MR (2007) Low-level light stimulates excisional wound healing in mice. Lasers Surg Med 39:706–715PubMedCrossRefGoogle Scholar
  11. 11.
    Passarella S, Casamassima E, Molinari S, Pastore D, Quagliariello E, Catalano IM, Cingolani A (1984) Increase of proton electrochemical potential and ATP synthesis in rat liver mitochondria irradiated in vitro by helium–neon laser. FEBS Lett 175:95–99PubMedCrossRefGoogle Scholar
  12. 12.
    Wu C, Yue Y, Deng X, Hua W, Gao Z (2004) Investigation on the synergetic effect between anatase and rutile nanoparticles in gas-phase photocatalytic oxidations. Catal Today 93–95:863–869CrossRefGoogle Scholar
  13. 13.
    Landau Z, Migdal M, Lipovsky A, Lubart R (2011) Visible light-induced healing of diabetic or venous foot ulcers: a placebo-controlled double-blind study. Photomed Laser Surg 29:399–404PubMedCrossRefGoogle Scholar
  14. 14.
    Abramovitch-Gottlib L, Gross T, Naveh D, Geresh S, Rosenwaks S, Bar I, Vago R (2005) Low level laser irradiation stimulates osteogenic phenotype of mesenchymal stem cells seeded on a three-dimensional biomatrix. Lasers Med Sci 20:138–146PubMedCrossRefGoogle Scholar
  15. 15.
    Soleimani M, Abbasnia E, Fathi M, Sahraei H, Fathi Y, Kaka G (2012) The effects of low-level laser irradiation on differentiation and proliferation of human bone marrow mesenchymal stem cells into neurons and osteoblasts-an in vitro study. Lasers Med Sci 27:423–430PubMedCrossRefGoogle Scholar
  16. 16.
    Alghamdi KM, Kumar A, Moussa NA (2012) Low-level laser therapy: a useful technique for enhancing the proliferation of various cultured cells. Lasers Med Sci 27:237–249PubMedCrossRefGoogle Scholar
  17. 17.
    Tuby H, Maltz L, Oron U (2007) Low-level laser irradiation (LLLI) promotes proliferation of mesenchymal and cardiac stem cells in culture. Lasers Surg Med 39:373–378PubMedCrossRefGoogle Scholar
  18. 18.
    Lubart R, Friedman H, Lavie R (2000) Photobiostimulation as a function of different wavelengths. Laser Ther 12:38–41CrossRefGoogle Scholar
  19. 19.
    Conlan MJ, Rapley JW, Cobb CM (1996) Biostimulation of wound healing by low-energy laser irradiation. A review. J Clin Periodontol 23:492–496PubMedCrossRefGoogle Scholar
  20. 20.
    Lavi R, Shainberg A, Friedmann H, Shneyvays V, Rickover O, Eichler M, Kaplan D, Lubart R (2003) Low energy visible light induces reactive oxygen species generation and stimulates an increase of intracellular calcium concentration in cardiac cells. J Biol Chem 278:40917–40922PubMedCrossRefGoogle Scholar
  21. 21.
    Friedmann H, Solodeeva I, Sinyakov M, Grossman N, Zurgil Z, Lubart R, Belosky S (1999) Photobiostimulation by visible light: involvement of hydrogen peroxide. Trends Photochem Photobiol 6:169–174Google Scholar
  22. 22.
    Davani S, Marandin A, Mersin N, Royer B, Kantelip B, Herve P, Etievent JP, Kantelip JP (2003) Mesenchymal progenitor cells differentiate into an endothelial phenotype, enhance vascular density, and improve heart function in a rat cellular cardiomyoplasty model. Circulation 108(Suppl 1):II253–II258PubMedGoogle Scholar
  23. 23.
    Lubart R (2009) A new method for stimulating the fertilizing capability of sperm cells. Recent Patents Biomed Eng 2:193–197CrossRefGoogle Scholar
  24. 24.
    Grossman N, Kleitman V, Meller J, Kaufmann R, Akgun N, Ruck A, Livneh E, Lubart R (2000) Role of PKC isozymes in low-power light-stimulated proliferation of cultured skin cells. Proc SPIE-Int Soc Opt Eng 4159:34–40CrossRefGoogle Scholar
  25. 25.
    Lubart R, Breitbart H, Sofer Y, Lavie R (1999) He-Ne irradiation of human spermatozoa: enhancement in hamster egg penetration. Laser Ther 11:171–176Google Scholar
  26. 26.
    Grossman N, Schneid N, Reuveni H, Halevy S, Lubart R (1998) 780 nm low power diode laser irradiation stimulates proliferation of keratinocyte cultures: Involvement of reactive oxygen species. Lasers Surg Med 22:212–218PubMedCrossRefGoogle Scholar
  27. 27.
    Lubart R, Friedmann H, Sinykov M, Grossman N (1995) Biostimulation of photosensitized fibroblasts by low incident levels of visible light energy. Laser Ther 7:101–106CrossRefGoogle Scholar
  28. 28.
    Grossman N, Reuveni H, Halevy S, Lubart R (1994) Visible-light promotes proliferation of normal skin cells. J Invest Dermatol 102:649Google Scholar
  29. 29.
    Lubart R, Friedmann H, Peled I, Grossman N (1993) Light effect on fibroblast proliferation. Laser Ther 5:55–57CrossRefGoogle Scholar
  30. 30.
    Eichler M, Lavi R, Shainberg A, Lubart R (2005) Flavins are source of visible-light-induced free radical formation in cells. Lasers Surg Med 37:314–319PubMedCrossRefGoogle Scholar
  31. 31.
    Ankri R, Friedman H, Savion N, Kotev-Emeth S, Breitbart H, Lubart R (2009) Visible light induces nitric oxide (NO) formation in sperm and endothelial cells. Lasers Surg Med 42:348–352CrossRefGoogle Scholar

Copyright information

© Springer-Verlag London Ltd 2012

Authors and Affiliations

  • Anat Lipovsky
    • 3
  • Uri Oron
    • 2
  • Aharon Gedanken
    • 3
  • Rachel Lubart
    • 1
    Email author
  1. 1.Departments of Chemistry and PhysicsBar-Ilan UniversityRamat-GanIsrael
  2. 2.Department of Zoology, The George S. Wise Faculty of Life SciencesTel-Aviv UniversityTel-AvivIsrael
  3. 3.Department of Chemistry, Kanbar Laboratory for Nanomaterials, Institute of Nanotechnology and Advanced MaterialsBar-Ilan UniversityRamat-GanIsrael

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