Lasers in Medical Science

, Volume 28, Issue 3, pp 981–987 | Cite as

Laser and LED phototherapies on angiogenesis

  • Ana Paula Cavalcanti de Sousa
  • Gardênia Matos Paraguassú
  • Nara Tayene Teixeira Silveira
  • José de Souza
  • Maria Cristina Teixeira Cangussú
  • Jean Nunes dos Santos
  • Antonio Luiz Barbosa PinheiroEmail author
Original Article


Angiogenesis is a key process for wound healing. There are few reports of LED phototherapy on angiogenesis, mainly in vivo. The aim of the present investigation was to evaluate histologically the angiogenesis on dorsal cutaneous wounds treated with laser (660 and 790 nm) or LEDs (700, 530, and 460 nm) in a rodent model. Twenty-four young adult male Wistar rats weighting between 200 and 250 g were used on the present study. Under general anesthesia, one excisional wound was created on the dorsum of each animal that were then randomly distributed into six groups with four animals each: G0—control; G1—laser λ660 nm (60 mW, ϕ ∼2 mm, 10 J/cm2); G2—laser λ790 nm (50 mW, ϕ ∼2 mm, 10 J/cm2); G3—LED λ700 ± 20 nm (15 mW, ϕ ∼16 mm, 10 J/cm2); G4—LED λ530 ± 20 nm (8 mW, ϕ ∼16 mm, 10 J/cm2); G5—LED λ460 ± 20 nm (22 mW, ϕ ∼16 mm, 10 J/cm2). Irradiation started immediately after surgery and was repeated every other day for 7 days. Animal death occurred at the eighth day after surgery. The specimens were removed, routinely processed to wax, cut and stained with HE. Angiogenesis was scored by blood vessel counting in the wounded area. Quantitative results showed that green LED (λ530 ± 20 nm), red LED (λ700 ± 20 nm), λ790 nm laser and λ660 nm laser caused significant increased angiogenesis when compared to the control group. It is concluded that both laser and LED light are capable of stimulating angiogenesis in vivo on cutaneous wounds and that coherence was not decisive on the outcome of the treatment.


Blood vessels Healing Light therapy 



The authors gratefully acknowledge the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for the financial support and PhD grant.


No competing financial interests exist.


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Copyright information

© Springer-Verlag London Ltd 2012

Authors and Affiliations

  • Ana Paula Cavalcanti de Sousa
    • 1
  • Gardênia Matos Paraguassú
    • 1
  • Nara Tayene Teixeira Silveira
    • 1
  • José de Souza
    • 2
    • 9
  • Maria Cristina Teixeira Cangussú
    • 3
    • 7
  • Jean Nunes dos Santos
    • 4
    • 8
  • Antonio Luiz Barbosa Pinheiro
    • 1
    • 5
    • 6
    Email author
  1. 1.Center of Biophotonics, School of DentistryFederal University of BahiaSalvadorBrazil
  2. 2.Institute of Health SciencesFederal University of BahiaSalvadorBrazil
  3. 3.Oral Epidemiology and Pediatric DentistryFederal University of BahiaSalvadorBrazil
  4. 4.Laboratory of Surgical Pathology, School of DentistryFederal University of BahiaSalvadorBrazil
  5. 5.Camilo Castelo Branco UniversitySão José dos CamposBrazil
  6. 6.Nacional Institute of Optics and PhotonicsSão CarlosBrazil
  7. 7.Dental Public Health, School of DentistryFederal University of BahiaSalvadorBrazil
  8. 8.Oral Pathology, School of DentistryFederal University of BahiaSalvadorBrazil
  9. 9.Laboratory of Neurosciences, Institute of Health SciencesFederal University of BahiaSalvadorBrazil

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