Lasers in Medical Science

, Volume 27, Issue 1, pp 153–160 | Cite as

The effect of different enamel surface treatments on the microleakage of fissure sealants

  • Ozgul BayginEmail author
  • Fatih Mehmet Korkmaz
  • Tamer Tüzüner
  • Mehmet Tanriver
Original Article


The aim of this study was to evaluate the effects of different techniques of surface treatment on the microleakage of a fissure sealant in molar teeth. A total of 50 freshly extracted noncarious human third molars were randomly assigned to one of five groups. Occlusal fissures were treated with one of the following: acid etching with 35% orthophosphoric acid (group 1); fissurotomy with a Fissurotomy Micro NTF metal bur (group 2); laser etching with an Er,Cr:YSGG laser at 2 W and 20 Hz (group 3); laser etching with an Er,Cr:YSGG laser at 2 W and 40 Hz (group 4); and air abrasion for 20 s with 30-µm Al2O3 particles via a CoJet Prep device (group 5). After surface pretreatment, a resin-based sealant was applied to the fissures. The sample teeth were subjected to thermocycling and stored in distilled water at 37°C for 1 month. Following immersion in 0.5% basic fuchsin solution for 24 h, three buccolingual slices of each sample tooth were scored under a stereomicroscope, and the morphological appearance of the area between the enamel surface and fissure sealant was examined under a scanning electron microscope. The Kruskal-Wallis test and one-way ANOVA revealed significant differences in marginal leakage, as follows: group 1 showed significantly lower scores than groups 2 and 5, the scores of groups 1, 3 and 4 were not significantly different, and group 2 showed significantly higher scores than groups 3 and 4. Laser irradiation, the metal bur, and the CoJet Prep device did not eliminate the need for acid etching of the enamel prior to placement of a fissure sealant. Laser etching at 2 W (20 Hz or 40 Hz) may be an alternative to conventional acid-etching.


Er,Cr:YSGG laser CoJet Prep Fissurotomy bur Etching Fissure sealant Microleakage 


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Copyright information

© Springer-Verlag London Ltd 2011

Authors and Affiliations

  • Ozgul Baygin
    • 1
    Email author
  • Fatih Mehmet Korkmaz
    • 2
  • Tamer Tüzüner
    • 1
  • Mehmet Tanriver
    • 1
  1. 1.Department of Pediatric Dentistry, Faculty of DentistryKaradeniz Technical UniversityTrabzonTurkey
  2. 2.Department of Prosthodontics, Faculty of DentistryKaradeniz Technical UniversityTrabzonTurkey

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