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Lasers in Medical Science

, Volume 26, Issue 5, pp 707–717 | Cite as

Low-level laser irradiation treatment reduces CCL2 expression in rat rheumatoid synovia via a chemokine signaling pathway

  • Lin Zhang
  • Jian Zhao
  • Noboru Kuboyama
  • Yoshimitsu AbikoEmail author
Original Article

Abstract

Rheumatoid arthritis (RA) is an inflammatory joint disorder whose progression leads to the destruction of cartilage and bone. Although low-level laser irradiation (LLLI) is currently being evaluated for the treatment of RA, the molecular mechanisms underlying its effectiveness remain unclear. To investigate possible LLLI-mediated antiinflammatory effects, we utilized a collagen-induced arthritis (CIA) rat model and analyzed gene expression profiles in the synovial membranes of the knee joint. Total RNA was isolated from the synovial membrane tissue of the joints of untreated CIA rats or CIA rats treated with LLLI (830 nm Ga-Al-As diode), and gene expression profiles were analyzed by DNA microarray (41,000 rat genes), coupled with Ingenuity pathways analysis (IPA). DNA microarray analysis showed that CCL2 gene expression was increased in CIA tissue, and that LLLI treatment significantly decreased CIA-induced CCL2 mRNA levels. IPA revealed that chemokine signal pathways were involved in the activation of CCL2 production. These microarray data were further validated using real-time PCR and reverse transcription PCR. Immunohistochemistry confirmed that CCL2 production was decreased in CIA rats treated with LLLI. These findings suggest that decreased CCL2 expression may be one of the mechanisms involved in LLLI-mediated RA inflammation reduction.

Keywords

Rheumatoid arthritis Rat joint CCL2 DNA microarray Low-level laser irradiation 

Notes

Acknowledgments

This study was supported in part by the “Academic Frontier” project for private Universities, a matching fund subsidy from the Ministry of Education, Culture, Sports, Science and Technology 2007–2011, and by a Grant-in-Aid for Scientific Research from the Japanese Society for the Promotion of Science (B21390497).

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Copyright information

© Springer-Verlag London Ltd 2011

Authors and Affiliations

  • Lin Zhang
    • 1
  • Jian Zhao
    • 1
  • Noboru Kuboyama
    • 2
  • Yoshimitsu Abiko
    • 1
    Email author
  1. 1.Department of Biochemistry and Molecular BiologyNihon University School of Dentistry at MatsudoMatsudoJapan
  2. 2.Department of Oral Molecular PharmacologyNihon University School of Dentistry at MatsudoMatsudoJapan

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