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Lasers in Medical Science

, Volume 26, Issue 3, pp 359–367 | Cite as

The effect of a single episode of antimicrobial photodynamic therapy in the treatment of experimental periodontitis. Microbiological profile and cytokine pattern in the dog mandible

  • Rafael Ramos de Oliveira
  • Arthur Belém NovaesJr.
  • Gustavo P. Garlet
  • Raphael F. de Souza
  • Mário TabaJr.
  • Sandra Sato
  • Sérgio L. S. de Souza
  • Daniela B. Palioto
  • Márcio F. M. Grisi
  • Magda Feres
Original Article

Abstract

The purpose of this study was to evaluate the effect of a single application of antimicrobial photodynamic therapy (aPDT) on microbiological profile and cytokine pattern in dogs. Periodontal disease was induced by placing 3.0 silk ligatures around the mandibular pre-molars bilaterally during 8 weeks. The dogs were randomly treated with aPDT using a dye/laser system, scaling and root planning (SRP), or with the association of treatments (SRP + aPDT). Plaque samples were collected at baseline, 1, 3, and 4 weeks, and the mean counts of 40 species were determined using DNA-DNA hybridization. Gingival biopsies were removed and the expression of tumor necrosis factor alpha (TNF-α), receptor activator of NF-kB ligand (RANKL), osteoprotegerin (OPG), matrix metalloproteinase (MMP-1), interleukin (IL) 6, IL-10 and total bacterial load by analysis of 16 S rRNA gene were evaluated through real-time PCR. The results shows that the levels of the majority of the species were reduced 1 week post-therapy for all treatments, however, an increase in counts of Prevotella intermedia (p = 0.00), Prevotella. nigrescens (p = 0.00) and Tannerella forsythia (p = 0.00) was observed for aPDT and SRP + aPDT. After 4 weeks, a regrowth of Porphyromonas gingivalis (p = 0.00) and Treponema denticola (p = 0.00), was observed for all treatments. Also, a strikingly reduction of counts on counts of Aggregatibacter actinomycetemcomitans was observed for the aPDT (p = 0.00). For the cytokine pattern, the results were similar for all treatments, and a reduction in the expression of cytokines and bacterial load was observed throughout the study. Our results suggest that SRP, aPDT in a single application, and SRP + aPDT affects different bacterial species and have similar effects on the expression of cytokines evaluated during the treatment of ligature-induced periodontitis.

Keywords

Photodynamic therapy Photosensitizing agents Cytokines Animal studies 

Notes

Acknowledgements

The diode laser, photosensitizer, and fiber optic applicator used in this study were donated by Helbo Photodynamic Systems, Grieskirchen, Austria. This study was in part supported by FAPESP (Grant # 2005/05600774-3 and 2005/60775-0). The authors report no conflicts of interest related to this study.

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Copyright information

© Springer-Verlag London Ltd 2010

Authors and Affiliations

  • Rafael Ramos de Oliveira
    • 1
  • Arthur Belém NovaesJr.
    • 1
    • 5
  • Gustavo P. Garlet
    • 2
  • Raphael F. de Souza
    • 3
  • Mário TabaJr.
    • 1
  • Sandra Sato
    • 3
  • Sérgio L. S. de Souza
    • 1
  • Daniela B. Palioto
    • 1
  • Márcio F. M. Grisi
    • 1
  • Magda Feres
    • 4
  1. 1.Department of Bucco-Maxillo-Facial Surgery and Traumatology and Periodontology, School of Dentistry of Ribeirão PretoUniversity of São PauloRibeirão PretoBrazil
  2. 2.Department of Biological Sciences, School of Dentistry of BauruUniversity of São PauloBauruBrazil
  3. 3.Department of Dental Materials and Prostheses, School of Dentistry of Ribeirão PretoUniversity of São PauloRibeirão PretoBrazil
  4. 4.Department of Periodontology, Dental Research DivisionGuarulhos UniversityGuarulhosBrazil
  5. 5.Faculdade de Odontologia de Ribeirão PretoUniversidade de São PauloRibeirão PretoBrazil

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