Lasers in Medical Science

, Volume 26, Issue 3, pp 335–340 | Cite as

Effects of low-level laser therapy on expression of TNF-α and TGF-β in skeletal muscle during the repair process

  • Raquel Agnelli Mesquita-Ferrari
  • Manoela Domingues Martins
  • Jose Antônio SilvaJr.
  • Tatiana Dias da Silva
  • Roberto Farina Piovesan
  • Vanessa Christina Santos Pavesi
  • Sandra Kalil Bussadori
  • Kristianne Porta Santos Fernandes
Original Article


The aim of the present study was to determine the effect of low-level laser therapy (LLLT) on the expression of TNF-α and TGF-β in the tibialis anterior muscle of rats following cryoinjury. Muscle regeneration involves cell proliferation, migration and differentiation and is regulated by growth factors and cytokines. A growing body of evidence suggests that LLLT promotes skeletal muscle regeneration by reducing the duration of acute inflammation and accelerating tissue repair. Adult male Wistar rats (n = 35) were randomly divided into three groups: control group (no lesion, untreated, n = 5), cryoinjury without LLLT group (n = 15), and cryoinjury with LLLT group (n = 15). The injured region was irradiated three times a week using an AlGaInP laser (660 nm; beam spot 0.04 cm2, output power 20 mW, power density 500 mW/cm2, energy density 5 J/cm2, exposure time 10 s). Muscle remodeling was evaluated at 1, 7 and 14 days (long-term) following injury. The muscles were removed and total RNA was isolated using TRIzol reagent and cDNA synthesis. Real-time polymerase chain reactions were performed using TNF-α and TGF-β primers; GAPDH was used to normalize the data. LLLT caused a decrease in TNF-α mRNA expression at 1 and 7 days following injury and in TGF-β mRNA expression at 7 days following cryoinjury in comparison to the control group. LLLT modulated cytokine expression during short-term muscle remodeling, inducing a decrease in TNF-α and TGF-β.


Laser Low-level laser therapy Myoblasts TNF-α TGF-β 



The authors would like to thank UNINOVE for financial support.

Conflicts of interest

The authors declare that there were no conflicting financial interests.


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Copyright information

© Springer-Verlag London Ltd 2010

Authors and Affiliations

  • Raquel Agnelli Mesquita-Ferrari
    • 1
  • Manoela Domingues Martins
    • 2
  • Jose Antônio SilvaJr.
    • 1
  • Tatiana Dias da Silva
    • 3
  • Roberto Farina Piovesan
    • 3
  • Vanessa Christina Santos Pavesi
    • 4
  • Sandra Kalil Bussadori
    • 1
  • Kristianne Porta Santos Fernandes
    • 1
  1. 1.Departamento de Pós Graduação, Mestrado em Ciências da ReabilitaçãoUniversidade Nove de Julho – UNINOVESão PauloBrazil
  2. 2.Dentistry SchoolUniversidade Federal do Rio Grande do Sul-UFRGSSão PauloBrazil
  3. 3.Departamento de Pos Graduacao, Mestrado em Ciencias da ReabilitacaoSão PauloBrazil
  4. 4.Dentistry SchoolUniversidade Nove de Julho – UNINOVESão PauloBrazil

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