Lasers in Medical Science

, Volume 26, Issue 3, pp 325–333 | Cite as

Peroperative optical autofluorescence biopsy—verification of its diagnostic potential

  • Vitezslav DucháčEmail author
  • Jiri Zavadil
  • Jana Vránová
  • Tomas Jirásek
  • Jan Štukavec
  • Ladislav Horák
Original Article


The authors tested the diagnostic potential of the portable autofluorescence optical system that was developed in the preoperative evaluation of resection margins, and thus of the resection line safety in patients with low-positioned colorectal carcinoma. A total of 217 spectral measurements of the fluorescence properties of normal (117) and malignant (100) tissues in 19 patients with colorectal carcinoma were accomplished. The measured spectra thus acquired were then evaluated using logistic regression. Using the principal component method, the authors selected the 30 and 40 most significant wavelengths, respectively, which they then used to construct the logistic model. The model met the basic criteria of statistical significance. The classification power of the model was 79.7% (for 30 wavelengths) and 82.5% (for 40). Statistical discrimination was 0.88 and 0.91, respectively. These results confirm that the optical setup that we selected is suitable for the peroperative testing of the distal resection line. It is capable of differentiating with 90% confidence pathological tissue and thus of reliably guiding further histological processing.


Colon cancer Resection margins Optical biopsy Fluorescence 



We greatly appreciate the cooperation of S. Javorsky (*) who helped set up the fiber-optic system.

* Institute of Photonics and Electronics, AS CR


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Copyright information

© Springer-Verlag London Ltd 2010

Authors and Affiliations

  • Vitezslav Ducháč
    • 1
    Email author
  • Jiri Zavadil
    • 2
  • Jana Vránová
    • 3
  • Tomas Jirásek
    • 4
  • Jan Štukavec
    • 1
  • Ladislav Horák
    • 1
  1. 1.Surgical Clinic of the 3rd Medical FacultyCharles University, PraguePrague 10Czech Republic
  2. 2.Institute of Photonics and ElectronicsAS CRPrague 8Czech Republic
  3. 3.Department of Medical Biophysics and Informatics of the IIIrd Medical FacultyCharles UniversityPrague 10Czech Republic
  4. 4.Department of Pathology of the IIIrd Medical FacultyCharles UniversityPrague 10Czech Republic

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