Lasers in Medical Science

, Volume 25, Issue 5, pp 743–748

Laser light combined with a photosensitizer may eliminate methicillin-resistant strains of Staphylococcus aureus

  • Khalil I. Hajim
  • Dhuha S. Salih
  • Yasemin Z. Rassam
Original Article

DOI: 10.1007/s10103-010-0803-z

Cite this article as:
Hajim, K.I., Salih, D.S. & Rassam, Y.Z. Lasers Med Sci (2010) 25: 743. doi:10.1007/s10103-010-0803-z

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of hospital acquired infection throughout the world especially in wound and burn infections, pneumonia, septicaemia and endocarditis. We describe the effect of a HeNe laser in combination with a TBO dye on the viability of MRSA. A total of 34 isolates of S. aureus were obtained from 100 patients suffering from burns or wounds and from the nasal vestibulum of medical and nonmedical staff as carriers; eight isolates were methicillin-resistant. The isolates were exposed for 5, 10 and 15 min to a HeNe laser at a wavelength of 632.8 nm and 7.5 mW output power in the presence of 50 μg/ml toluidune blue O photosensitizer. The viable count was substantially decreased as determined by the plate count method for the three exposure times, with 100% killing with the 15-min exposure time. No significant effect was observed on MRSA isolates exposed to the laser alone. So MRSA was completely eradicated following 15 min exposure to a 632.8-nm HeNe laser in the presence of 50 μg/ml toluidune blue O photosensitizer under in vitro conditions.

Keywords

Photodynamic therapy MRSA Pathogenesis Nosocomial infection 

Copyright information

© Springer-Verlag London Ltd 2010

Authors and Affiliations

  • Khalil I. Hajim
    • 1
  • Dhuha S. Salih
    • 2
  • Yasemin Z. Rassam
    • 1
  1. 1.Institute of Laser for Post Graduate StudiesUniversity of BaghdadBaghdadIraq
  2. 2.College of Science, Biology DepartmentUniversity of BaghdadBaghdadIraq

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