Advertisement

Lasers in Medical Science

, Volume 26, Issue 4, pp 439–444 | Cite as

Relationship between laser fluorescence and bacterial invasion in arrested dentinal carious lesions

  • Yukiteru IwamiEmail author
  • Hiroko Yamamoto
  • Mikako Hayashi
  • Shigeyuki Ebisu
Original Article

Abstract

This study investigated the relationship between caries assessment using a laser fluorescence device (DIAGNOdent), and bacterial invasion in arrested carious dentin detected by polymerase chain reaction (PCR). The ten extracted human molars used in this study had black or dark brown, hard occlusal carious lesions, and were found to be only weakly stained or unstained with a caries detector dye of 1% acid red in propylene glycol. In those extracted human molars, dentin was removed in the direction of the pulp chamber at 150-μm intervals. During each removal (104 sections in total), the dentin surface was assessed with DIAGNOdent, and a dentinal tissue sample was taken with a round bur. Bacterial DNA of each tissue sample was examined using PCR and primers based on the nucleotide sequence of a conserved region of bacterial 16S rDNA. Rates of bacterial detection increased as the DIAGNOdent values increased. When the DIAGNOdent values were <10, the rate of bacterial detection was 0%. The area under the receiver operating characteristic curve of the DIAGNOdent values was 0.87. These results indicate that the DIAGNOdent values of arrested dentinal carious lesion were closely related to the rates of bacterial detection.

Keywords

Laser fluorescence device Arrested caries Bacterial infection Polymerase chain reaction 

Notes

Acknowledgments

This work was partly supported by a Grant-in-Aid for Scientific Research (C) (19592199) from the Japan Society for the Promotion of Science.

References

  1. 1.
    Tyas MJ, Anusavice KJ, Frencken JE, Mount GJ (2000) Minimal intervention dentistry—a review. FDI Commission Project 1–97. Int Dent J 50:1–12PubMedGoogle Scholar
  2. 2.
    Mount GJ, Ngo H (2000) Minimal intervention: a new concept for operative dentistry. Quintessence Int 31:527–533PubMedGoogle Scholar
  3. 3.
    Miller WA, Massler M (1962) Permeability and staining of active and arrested lesions in dentine. Br Dent J 112:187–197Google Scholar
  4. 4.
    Okuse K (1964) Relationship between hardness, discoloration and organismal invasion in carious dentin. J Stomatol Soc Jpn 31:187–200Google Scholar
  5. 5.
    Sano H (1987) Relationship between caries detector staining and structural characteristics of carious dentin. J Stomatol Soc Jpn 54:241–270Google Scholar
  6. 6.
    Fukushima M (1981) Adhesive resin penetration into carious dentin. J Stomatol Soc Jpn 48:362–385Google Scholar
  7. 7.
    Lundeen TF, Roberson TM (1995) Caries diagnosis and preventive treatment. In: Sturdevant CM, Roberson TM, Heymann JR, Sturdevant JR (eds) The art and science of operative dentistry, 3rd edn. Mosby, St. Louis, pp 100–106Google Scholar
  8. 8.
    Banerjee A, Watson TF, Kidd EAM (2000) Dentine caries: take it or leave it? Dent Update 27:272–276PubMedGoogle Scholar
  9. 9.
    Iwami Y, Hayashi N, Yamamoto H, Hayashi M, Takeshige F, Ebisu S (2007) Evaluating the objectivity of caries removal with a caries detector dye using color evaluation and PCR. J Dent 35:749–754. doi: 10.1016/j.jdent.2007.06.004 PubMedCrossRefGoogle Scholar
  10. 10.
    Shi XQ, Welander U, Angmar-Månsson B (2000) Occlusal caries detection with KaVo DIAGNOdent and radiography: an in vitro comparison. Caries Res 34:151–158. doi: 10.1159/000016583 PubMedCrossRefGoogle Scholar
  11. 11.
    Mendes FM, Hissadomi M, Imparato JCP (2004) Effects of drying time and the presence of plaque on the in vitro performance of laser fluorescence in occlusal caries of primary teeth. Caries Res 38:104–108. doi: 10.1159/000075933 PubMedCrossRefGoogle Scholar
  12. 12.
    Rodrigues JA, Diniz MB, Josgrilberg EB, Cordeiro RCL (2009) In vitro comparison of laser fluorescence performance with visual examination for detection of occlusal caries in permanent and primary molars. Lasers Med Sci 24:501–506. doi: 10.1007/s10103-008-0552-4 PubMedCrossRefGoogle Scholar
  13. 13.
    Lussi A, Megert B, Longbottom C, Reich E, Francescut P (2001) Clinical performance of a laser fluorescence device for detection of occlusal caries lesions. Eur J Oral Sci 109:14–19. doi: 10.1034/j.1600-0722.2001.109001014.x PubMedCrossRefGoogle Scholar
  14. 14.
    Sundström F, Frediksson K, Montan S, Hafström-Björkman U, Ström J (1985) Laser-induced fluorescence from sound and carious tooth substance: spectroscopic studies. Swed Dent J 9:71–81PubMedGoogle Scholar
  15. 15.
    Iwami Y, Shimizu A, Narimatsu M, Hayashi M, Takeshige F, Ebisu S (2004) Relationship between bacterial infection and evaluation using a laser fluorescence device, DIAGNOdent. Eur J Oral Sci 112:419–423PubMedCrossRefGoogle Scholar
  16. 16.
    Lennon AM, Buchalla W, Switalski L, Stookey GK (2002) Residual caries detection using visible fluorescence. Caries Res 36:315–319. doi: 10.1159/000065956 PubMedCrossRefGoogle Scholar
  17. 17.
    Boston DW, Sauble JE (2005) Evaluation of laser fluorescence for differentiating caries dye-stainable versus caries dye-unstainable dentin in carious lesions. Am J Dent 18:351–354PubMedGoogle Scholar
  18. 18.
    Yazaci R, Baseren M, Gokalp S (2005) The in vitro performance of laser fluorescence and caries-detector dye for detecting residual carious dentin during tooth preparation. Quintessence Int 36:417–422Google Scholar
  19. 19.
    Calvalho JC, Thylstrup A, Ekstrand KR (1992) Results after 3 years of non-operative occlusal caries treatment of erupting permanent first molars. Commun Dent Oral Epidemiol 20:187–192CrossRefGoogle Scholar
  20. 20.
    Johansen E, Papas A, Fong W, Olsen TO (1987) Remineralization of carious lesions in elderly patients. Gerodont 3:47–50Google Scholar
  21. 21.
    Kidd EA, Ricketts DN, Beighton D (1996) Criteria for caries removal at the enamel-dentine junction: a clinical and microbiological study. Br Dent J 180:287–291PubMedCrossRefGoogle Scholar
  22. 22.
    Iwami Y, Yamamoto H, Ebisu S (2000) A new electrical method for detecting marginal leakage of in vitro resin restorations. J Dent 28:241–247PubMedCrossRefGoogle Scholar
  23. 23.
    Iwami Y, Shimizu A, Narimatsu M, Kinomoto Y, Ebisu S (2005) The relationship between the color of carious dentin stained with a caries detector dye and bacterial infection. Oper Dent 30:83–89PubMedGoogle Scholar
  24. 24.
    Iwami Y, Hayashi N, Takeshige F, Ebisu S (2008) Relationship between the color of carious dentin with varying lesion activity, and bacterial detection. J Dent 36:143–151. doi: 10.1016/J.JDENT.2007.11.012 PubMedCrossRefGoogle Scholar
  25. 25.
    Nadkarni MA, Martin FE, Jacques NA, Hunter N (2002) Determination of bacterial load by real-time PCR using a broad-range (universal) probe and primer set. Microbiol 148(Pt1):257–266Google Scholar
  26. 26.
    Bjorndal L, Larsen T (2000) Changes in the cultivable flora in deep carious lesions following a stepwise excavation procedure. Caries Res 34:502–508PubMedCrossRefGoogle Scholar
  27. 27.
    Hoshino E (1985) Predominant obligate anaerobes in human caries dentin. J Dent Res 64:1195–1198PubMedCrossRefGoogle Scholar
  28. 28.
    Aihara H, Kimishita T, Nara Y, Katsuumi I (2008) Mutual relation among bond strength of resin adhesive systems to carious dentine, DIAGNOdent™ value and dyeing degree with Caries Detector®. Jpn J Conserv Dent 51:191–202Google Scholar
  29. 29.
    Dreizen S, Spies TD (1950) A note on the production of a yellow-brown pigment in the organic matrices of noncarious human teeth by oral lactobacilli. Oral Surg Oral Med Oral Path 3:686–691PubMedCrossRefGoogle Scholar
  30. 30.
    Dreizen S, Gilly EJ, Mosny JJ, Spies TD (1957) Experimental observation on melanoidin formation in human carious teeth. J Dent Res 36:233–236PubMedCrossRefGoogle Scholar
  31. 31.
    Hibst R, Paulus R, Lussi A (2001) Detection of occlusal caries by laser fluorescence: basic and clinical investigations. Med Laser App 16:205–213CrossRefGoogle Scholar
  32. 32.
    Shi XQ, Tranæus S, Angmar-Månsson B (2001) Validation of DIAGNOdent for quantification of smooth-surface caries: an in vitro study. Acta Odontol Scand 59:74–78. doi: 10.1080/000163501750157153 PubMedCrossRefGoogle Scholar
  33. 33.
    Ho TFT, Smales RJ, Fang DTS (1999) A 2-year clinical study of two glass ionomer cements used in the atraumatic restorative treatment (ART) technique. Commun Dent Oral Epidemiol 27:195–201. doi: 10.1111/J.1600-0528.1999.tb02010.x CrossRefGoogle Scholar
  34. 34.
    Mertz-Fairhurst EJ, Curtis JW Jr, Ergle JW, Rueggeberg FA, Adair SM (1998) Ultraconservative and cariostatic sealed restorations: results at year 10. J Am Dent Assoc 129:55–66PubMedGoogle Scholar
  35. 35.
    Lager A, Thornqvist E, Ericson D (2003) Cultivatable bacteria in dentine after caries excavation using rose-bur or Carisolv. Caries Res 37:206–211. doi: 10.1159/000070446 PubMedCrossRefGoogle Scholar
  36. 36.
    Lennon ÁM (2003) Fluorescence-aided caries excavation (FACE) compared with conventional method. Oper Dent 28:341–345PubMedGoogle Scholar

Copyright information

© Springer-Verlag London Ltd 2010

Authors and Affiliations

  • Yukiteru Iwami
    • 1
    Email author
  • Hiroko Yamamoto
    • 1
  • Mikako Hayashi
    • 1
  • Shigeyuki Ebisu
    • 1
  1. 1.Department of Restorative Dentistry and EndodontologyOsaka University Graduate School of DentistryOsakaJapan

Personalised recommendations