Lasers in Medical Science

, Volume 25, Issue 5, pp 705–710 | Cite as

Antimicrobial effect of photodynamic therapy using a highly pure chlorin e6

Original Article

Abstract

The aim of the present study is to evaluate the antimicrobial effect of photodynamic therapy (PDT) using a highly pure chlorin e6 (Ce6), against various pathogenic bacteria. To examine the antimicrobial effect of Ce6-mediated PDT against Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Salmonella enterica serovar Typhimurium, inhibition zone formation, CFU quantification, and bacterial viability were evaluated. Inhibition zone analysis showed that Ce6-mediated PDT is very effective to inhibit the growth of S. aureus and P. aeruginosa, but has only minor effect to E. coli and S. Typhimurium, which was dependent on the energy density of laser and dose of Ce6. Ce6-mediated PDT also nearly inhibited the colony formation of S. aureus and P. aeruginosa, and partially inhibited that of E. coli and S. Typhimurium. In addition, the number of viable bacteria decreased greatly after PDT application with LS-chlorin e6 of 10 μM and laser and energy density of 20 J/cm2. These results show that Ce6-mediated PDT can be an effective alternative for antimicrobial treatment.

Keywords

Photodynamic therapy Chlorin e6 Inhibition zone formation Bacterial viability Antimicrobial treatment 

Notes

Acknowledgements

This study was supported by a grant from the Korea Healthcare Technology R&D Project, Ministry for Health, Welfare & Family Affairs, Republic of Korea (A080160).

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Copyright information

© Springer-Verlag London Ltd 2010

Authors and Affiliations

  • Jong-Hwan Park
    • 1
  • Yeon-Hee Moon
    • 2
  • Iel-Soo Bang
    • 3
  • Yong-Chul Kim
    • 4
  • Soo-A Kim
    • 5
  • Sang-Gun Ahn
    • 2
  • Jung-Hoon Yoon
    • 2
  1. 1.Department of Biochemistry, College of MedicineKonyang UniversityDaejeonRepublic of Korea
  2. 2.Department of Pathology, School of DentistryChosun UniversityGwangjuRepublic of Korea
  3. 3.Microbiology & Immunology, School of DentistryChosun UniversityGwangjuRepublic of Korea
  4. 4.Department of Life ScienceGISTGwangjuRepublic of Korea
  5. 5.Department of Biochemistry, College of Oriental MedicineDongguk UniversityGyungjuRepublic of Korea

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