Lasers in Medical Science

, Volume 25, Issue 5, pp 705–710 | Cite as

Antimicrobial effect of photodynamic therapy using a highly pure chlorin e6

Original Article


The aim of the present study is to evaluate the antimicrobial effect of photodynamic therapy (PDT) using a highly pure chlorin e6 (Ce6), against various pathogenic bacteria. To examine the antimicrobial effect of Ce6-mediated PDT against Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Salmonella enterica serovar Typhimurium, inhibition zone formation, CFU quantification, and bacterial viability were evaluated. Inhibition zone analysis showed that Ce6-mediated PDT is very effective to inhibit the growth of S. aureus and P. aeruginosa, but has only minor effect to E. coli and S. Typhimurium, which was dependent on the energy density of laser and dose of Ce6. Ce6-mediated PDT also nearly inhibited the colony formation of S. aureus and P. aeruginosa, and partially inhibited that of E. coli and S. Typhimurium. In addition, the number of viable bacteria decreased greatly after PDT application with LS-chlorin e6 of 10 μM and laser and energy density of 20 J/cm2. These results show that Ce6-mediated PDT can be an effective alternative for antimicrobial treatment.


Photodynamic therapy Chlorin e6 Inhibition zone formation Bacterial viability Antimicrobial treatment 



This study was supported by a grant from the Korea Healthcare Technology R&D Project, Ministry for Health, Welfare & Family Affairs, Republic of Korea (A080160).


  1. 1.
    Seal JB, Moreira B, Bethel CD, Daum RS (2003) Antimicrobial resistance in Staphylococcus aureus at the University of Chicago Hospitals: a 15-year longitudinal assessment in a large university-based hospital. Infect Control Hosp Epidemiol 24:403–408CrossRefPubMedGoogle Scholar
  2. 2.
    Cerveny KE, DePaola A, Duckworth DH, Gulig PA (2002) Phage therapy of local and systemic disease caused by Vibrio vulnificus in iron-dextran-treated mice. Infect Immun 70:6251–6262CrossRefPubMedGoogle Scholar
  3. 3.
    Sajjan US, Tran LT, Sole N, Rovaldi C, Akiyama A, Friden PM, Forstner JF, Rothstein DM (2001) P-113D, an antimicrobial peptide active against Pseudomonas aeruginosa, retains activity in the presence of sputum from cystic fibrosis patients. Antimicrob Agents Chemother 45:3437–3444CrossRefPubMedGoogle Scholar
  4. 4.
    Wainwright M (1998) Photodynamic antimicrobial chemotherapy (PACT). J Antimicrob Chemother 42:13–28CrossRefPubMedGoogle Scholar
  5. 5.
    Kossodo S, LaMuraglia GM (2001) Clinical potential of photodynamic therapy in cardiovascular disorders. Am J Cardiovasc Drugs 1:15–21CrossRefPubMedGoogle Scholar
  6. 6.
    Ahmad S, Bearelly S, Stinnett SS, Cooney MJ, Fekrat S (2008) Photodynamic therapy for predominantly hemorrhagic lesions in neovascular age-related macular degeneration. Am J Ophthalmol 145:1052–1057CrossRefPubMedGoogle Scholar
  7. 7.
    Sibata CH, Colussi VC, Oleinick NL, Kinsella TJ (2001) Photodynamic therapy in oncology. Expert Opin Pharmacother 2:917–927CrossRefPubMedGoogle Scholar
  8. 8.
    Silva JN, Filipe P, Morliere P, Maziere JC, Freitas JP, Gomes MM, Santus R (2008) Photodynamic therapy: dermatology and ophthalmology as main fields of current applications in clinic. Biomed Mater Eng 18:319–327PubMedGoogle Scholar
  9. 9.
    Trauner KB, Hasan T (1996) Photodynamic treatment of rheumatoid and inflammatory arthritis. Photochem Photobiol 64:740–750CrossRefPubMedGoogle Scholar
  10. 10.
    Phoenix DA, Sayed Z, Hussain S, Harris F, Wainwright M (2003) The phototoxicity of phenothiazinium derivatives against Escherichia coli and Staphylococcus aureus. FEMS Immunol Med Microbiol 39:17–22CrossRefPubMedGoogle Scholar
  11. 11.
    Hamblin MR, Hasan T (2004) Photodynamic therapy a new antimicrobial approach to infectious disease? Photochem Photobiol Sci 3:436–450CrossRefPubMedGoogle Scholar
  12. 12.
    Peloi LS, Soares RR, Biondo CE, Souza VR, Hioka N, Kimura E (2008) Photodynamic effect of light-emitting diode light on cell growth inhibition induced by methylene blue. J Biosci 33:231–237CrossRefPubMedGoogle Scholar
  13. 13.
    Kostenich GA, Zhuravkin IN, Zhavrid EA (1994) Experimental grounds for using chlorin e6 in the photodynamic therapy of malignant tumors. J Photochem Photobiol B 22:211–217CrossRefPubMedGoogle Scholar
  14. 14.
    Moon YH, Kwon SM, Kim HJ, Jung KY, Park JH, Kim SA, Kim YC, Ahn SG, Yoon JH (2009) Efficient preparation of highly pure chlorin e6 and its photodynamic anti-cancer activity in rat tumor model. Oncol Rep 22:1085–1091PubMedGoogle Scholar
  15. 15.
    Demidova TN, Gad F, Zahra T, Francis KP, Hamblin MR (2005) Monitoring photodynamic therapy of localized infections by bioluminescence imaging of genetically engineered bacteria. J Photochem Photobiol B 81:15–25CrossRefPubMedGoogle Scholar
  16. 16.
    Malik ZA, Broughel D (2007) The importance of testing whole stool for Shiga toxin: a clinical and microbiological perspective. J Pak Med Assoc 57:265–266PubMedGoogle Scholar
  17. 17.
    Tegos GP, Anbe M, Yang C, Demidova TN, Satti M, Mroz P, Janjua S, Gad F, Hamblin MR (2006) Proteae-stable polycationic photosensitizer conjugates between polyethyleneimine and chlorin(e6) for broad-spectrum antimicrobial photoinactivation. Antimicrob Agents Chemother 50:1402–1410CrossRefPubMedGoogle Scholar
  18. 18.
    Hamblin MR, O’Donnell DA, Murthy N, Rajagopalan K, Michaud N, Sherwood ME, Hasan T (2002) Polycationic photosensitizer conjugates: effects of chain length and Gram classification on the photodynamic inactivation of bacteria. J Antimicrob Chemother 49:941–951CrossRefPubMedGoogle Scholar
  19. 19.
    Malik Z, Hanania J, Nitzan Y (1990) Bactericidal effects of photoactivated porphyrins—an alternative approach to antimicrobial drugs. J Photochem Photobiol B 5:281–293CrossRefPubMedGoogle Scholar
  20. 20.
    Bertoloni G, Salvato B, Dall’Acqua M, Vazzoler M, Jori G (1984) Hematoporphyrin-sensitized photoinactivation of Streptococcus faecalis. Photochem Photobiol 39:811–816CrossRefPubMedGoogle Scholar
  21. 21.
    Nitzan Y, Gutterman M, Malik Z, Ehrenberg B (1992) Inactivation of Gram-negative bacteria by photosensitized porphyrins. Photochem Photobiol 55:89–96CrossRefPubMedGoogle Scholar
  22. 22.
    Jori G, Fabris C, Soncin M, Ferro S, Coppellotti O, Dei D, Fantetti L, Chiti G, Roncucci G (2006) Photodynamic therapy in the treatment of microbial infections: basic principles and perspective applications. Laser Sur Med 38:468–481CrossRefGoogle Scholar

Copyright information

© Springer-Verlag London Ltd 2010

Authors and Affiliations

  • Jong-Hwan Park
    • 1
  • Yeon-Hee Moon
    • 2
  • Iel-Soo Bang
    • 3
  • Yong-Chul Kim
    • 4
  • Soo-A Kim
    • 5
  • Sang-Gun Ahn
    • 2
  • Jung-Hoon Yoon
    • 2
  1. 1.Department of Biochemistry, College of MedicineKonyang UniversityDaejeonRepublic of Korea
  2. 2.Department of Pathology, School of DentistryChosun UniversityGwangjuRepublic of Korea
  3. 3.Microbiology & Immunology, School of DentistryChosun UniversityGwangjuRepublic of Korea
  4. 4.Department of Life ScienceGISTGwangjuRepublic of Korea
  5. 5.Department of Biochemistry, College of Oriental MedicineDongguk UniversityGyungjuRepublic of Korea

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