Lasers in Medical Science

, Volume 25, Issue 4, pp 531–537 | Cite as

Activation of the extracellular signal-regulated kinase signal pathway by light emitting diode irradiation

  • Nobuhiko Komine
  • Kazuo Ikeda
  • Kaoru Tada
  • Noriyuki Hashimoto
  • Naotoshi Sugimoto
  • Katsuro Tomita
Original Article


Irradiation by light emitting diode (LED) promotes fibroblast proliferation and wound healing. However, its mechanism is still unknown. The purpose of this study was to clarify the mechanism of fibroblast proliferation by LED irradiation. Cultured NIH3T3 fibroblasts from normal mice were irradiated by LED with a center wavelength of 627 nm. LED irradiation was performed with an energy density of 4 J/cm2, at subculture and 24 h later. The expression of several growth factors and their receptors was analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR): platelet-derived growth factor (PDGF)-A, PDGF-B, and PDGF-C, transforming growth factor-beta (TGF-β), basic fibroblast growth factor (bFGF), PDGF-α receptor, and TGF-β receptor. Then, the activation of the extracellular signal-regulated kinase (ERK) pathway was examined by Western blotting with and without the PDGF receptor inhibitor. LED irradiation induced cell growth of NIH3T3 fibroblasts. The expression of PDGF-C had significantly increased in the irradiated group (P < 0.01). Although strong activation of the ERK pathway was observed in the irradiated group, its activation was completely suppressed by the PDGF receptor inhibitor. We concluded that LED irradiation promotes fibroblast proliferation by increasing autocrine production of PDGF-C and activating the ERK pathway through phosphorylation of the PDGF receptor.


Light emitting diode Extracellular signal-regulated kinase Growth factor Wound healing 


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Copyright information

© Springer-Verlag London Ltd 2010

Authors and Affiliations

  • Nobuhiko Komine
    • 1
  • Kazuo Ikeda
    • 2
  • Kaoru Tada
    • 1
  • Noriyuki Hashimoto
    • 1
  • Naotoshi Sugimoto
    • 3
  • Katsuro Tomita
    • 1
  1. 1.Department of Orthopaedic Surgery, School of MedicineKanazawa UniversityKanazawaJapan
  2. 2.Department of Orthopaedic SurgeryKanazawa Medical CenterKanazawaJapan
  3. 3.Department of Physiology, School of MedicineKanazawa UniversityKanazawaJapan

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