Histological findings in adipocytes when cellulite is treated with a variable-emission radiofrequency system
- 379 Downloads
Cellulite is a disorder of the subcutaneous fat layer and the overlying superficial skin. Recently, radiofrequency (RF) has been proposed as an effective treatment of cellulite; however, the mechanism through which the absorbed electrical energy acts on the tissue is still not fully clear. This study reports on the histological findings from biopsies taken immediately after a single RF treatment session, on cellulite located in the buttocks, with a novel technology called automatic multi-frequency and low impedance (AMFLI) RF. Tissue samples were stained with either haematoxylin and eosin (H&E), so that we could study the morphological findings, or with oil red O, to enable us to identify lipid deposits. The histological findings observed in biopsies taken after a single RF treatment showed changes in shape, size, and lipid content, as well as in cytoplasmic and nuclear morphology. After RF treatment adipocytes were more polyhedric, with irregular, degenerated membranes, with less or no lipid content and apoptotic changes. We postulate that RF treatment on cellulite produces a decrease in lipid content of cells as well as changes in the adipocyte membrane which will lead to cell rupture and the death and extrusion of lipid content out of the cell. Further studies are needed to characterise the nature of the extra-cellular lipid material that we have demonstrated with the oil red O stain in our biopsies.
KeywordsRadiofrequency Adipose tissue Cellulite Histology Red oil
On behalf of his co-authors and himself, M.A.T. declares no financial or other interest in the company and equipment mentioned in this study. The authors acknowledge receipt of a grant for this study from the Fundación Antoni de Gimbernat in whose 2007–2008 activities the subject matter of this article is registered.
- 9.del Pino E, Rosado RH, Azuela A, Guzman G, Arguelles D, Rodriguez C, Rosado GM (2006) Effect of controlled volumetric tissue heating with radiofrequency on cellulite and the subcutaneous tissue of the buttocks and thighs. J Drugs Dermatol 5:714–722Google Scholar
- 14.Hoffman AH, O’Connor JP, Grigg P (2007) Realignment of collagen fibers in mouse skin. Proceedings Book, FASCIA Research CongressGoogle Scholar
- 15.Trelles, MA, Levy, JL, Ghersetich, I (2008) Effects achieved on stretch marks by a nonfractional broadband infrared light system treatment. Aesthetic Plast Surg 32:523–530Google Scholar