Lasers in Medical Science

, Volume 24, Issue 4, pp 539–547 | Cite as

Effect of equal daily doses achieved by different power densities of low-level laser therapy at 635 nm on open skin wound healing in normal and corticosteroid-treated rats

  • Peter GálEmail author
  • Michal Mokrý
  • Boris Vidinský
  • Róbert Kilík
  • Filip Depta
  • Magdaléna Harakaľová
  • František Longauer
  • Štefan Mozeš
  • Ján Sabo
Original Article


Optimal parameters of low-level laser therapy (LLLT) for wound healing are still discussed. Hence, our study was aimed to compare effects of different power densities of LLLT at 635 nm in rats. Four, round, full-thickness, skin wounds were made on the backs of 48 rats that were divided into two groups (non-steroid laser-treated and steroid laser-treated). Three wounds were stimulated daily with a diode laser (daily dose 5 J/cm2) each with different power density (1 mW/cm2, 5 mW/cm2, and 15 mW/cm2), whereas the fourth wound served as a control. Two days, 6 days, and 14 days after surgery, eight animals from each group were killed and samples were removed for histological evaluation. In the non-steroid laser-treated rats, significant acceleration of epithelization and collagen synthesis 2 days and 6 days after surgery was observed in stimulated wounds. In steroid laser-treated rats, 2 days and 14 days after surgery, a decreased leucocyte/macrophage ratio and a reduction in the area of granulation tissue were recorded, respectively. In conclusion, LLLT, by the method we used, improved wound healing in the non-steroid laser-treated rats, but it was useless after corticosteroid treatment.


Daily dose of 5 J/cm2 Low-level laser therapy Wound healing Different power densities 



We thank Magdaléna Majnušová for preparing the histological sections. Also, we thank Marek Antol, Martin Novotný, Lenka Kostičová, and Eva Kožejová for their useful technical assistance. In addition, we thank Faiza Hussain for editorial help in preparing this manuscript. This study was partially supported by the Slovak Grant Agency of the Ministry of Education and Slovak Academy of Sciences (VEGA, 1/3361/06 and 1/4228/07). We are grateful to U.S. Steel Košice, Slovak Republic, for buying us the Olympus BX51 microscope.


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Copyright information

© Springer-Verlag London Ltd 2008

Authors and Affiliations

  • Peter Gál
    • 1
    Email author
  • Michal Mokrý
    • 1
  • Boris Vidinský
    • 1
  • Róbert Kilík
    • 2
  • Filip Depta
    • 1
  • Magdaléna Harakaľová
    • 1
  • František Longauer
    • 3
  • Štefan Mozeš
    • 4
  • Ján Sabo
    • 1
  1. 1.Department of Medical BiophysicsPavol Jozef Šafárik UniversityKošiceSlovak Republic
  2. 2.1st Department of SurgeryPavol Jozef Šafárik University and Louise Pasteur Faculty HospitalKošiceSlovak Republic
  3. 3.Department of Forensic MedicinePavol Jozef Šafárik UniversityKošiceSlovak Republic
  4. 4.Institute of Animal PhysiologySlovak Academy of SciencesKošiceSlovak Republic

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