Lasers in Medical Science

, Volume 24, Issue 3, pp 295–300 | Cite as

In vivo evaluation of laser fluorescence performance using different cut-off limits for occlusal caries detection

  • Michele Baffi Diniz
  • Jonas de Almeida Rodrigues
  • Andréia Bolzan de Paula
  • Rita de Cássia Loiola Cordeiro
Original article


The aim of this in vivo study was to evaluate the performance of laser fluorescence (LF) comparing different cut-off limits for occlusal caries detection. One hundred and thirty first permanent molars were selected. Visual examination and LF assessments were performed independently. The extent of caries was assessed after operative intervention. New cut-off limits were established and compared with those proposed by the manufacturer and by Lussi et al. (Eur J Oral Sci 109:14–19, 2001). Similar sensitivity and higher specificity was found at D2 (considering as disease only dentin caries) when the LF cut-off limits proposed by Lussi et al. and the new one were compared. At the D3 threshold (considering as disease only deep dentin caries), no statistically significant difference among the cut-off limits for sensitivity was found. However, the new cut-off limits showed higher specificity. The LF device provided good ability to detect dentin caries lesions. Furthermore, the new cut-off limits and the values proposed by Lussi et al. could be suggested for the in vivo detection of occlusal caries.


Occlusal caries DIAGNOdent Laser fluorescence In vivo 



The authors wish to thank Dr. Robert L. Karlinsey for the English corrections. This study was submitted to the Araraquara School of Dentistry, São Paulo State University, as a requirement for a Master’s degree.


  1. 1.
    Lussi A, Megert B, Longbottom C, Reich E, Francescut P (2001) Clinical performance of a laser fluorescence device for detection of occlusal caries lesion. Eur J Oral Sci 109:14–19CrossRefPubMedGoogle Scholar
  2. 2.
    Mejàre I, Källestål C, Stenlund H, Johansson H (1998) Caries development from 11 to 22 years of age: a prospective radiographic study. Prevalence and distribution. Caries Res 32:10–16CrossRefPubMedGoogle Scholar
  3. 3.
    Hannigan A, O’Mullane DM, Barry D, Schäfer F, Roberts AJ (2000) A caries susceptibility classification of tooth surfaces by survival time. Caries Res 34:103–108CrossRefPubMedGoogle Scholar
  4. 4.
    Pretty IA, Maupomé G (2004) A closer look at diagnosis in clinical dental practice: part 5. Emerging technologies for caries detection and diagnosis. J Can Dent Assoc 70:540–540iPubMedGoogle Scholar
  5. 5.
    Verdonschot EH, van der Veen MH (2002) Lasers in dentistry 2. Diagnosis of dental caries with lasers. Ned Tijdschr Tandheelkd 109:122–126PubMedGoogle Scholar
  6. 6.
    Hibst R, Paulus R, Lussi A (2001) Detection of occlusal caries by laser fluorescence. Basic and clinical investigations. Med Laser Appl 16:205–213Google Scholar
  7. 7.
    Sheehy EC, Brailsford SR, Kidd EA, Beighton D, Zoitopoulos L (2001) Comparison between visual examination and a laser fluorescence system for in vivo diagnosis of occlusal caries. Caries Res 35:421–426CrossRefPubMedGoogle Scholar
  8. 8.
    Young DA (2002) New caries detection technologies and modern caries management: merging the strategies. Gen Dent 50:320–331PubMedGoogle Scholar
  9. 9.
    Lussi A, Imwinkelried S, Pitts N, Longbottom C, Reich E (1999) Performance and reproducibility of a laser fluorescence system for detection of occlusal caries in vitro. Caries Res 33:261–266CrossRefPubMedGoogle Scholar
  10. 10.
    Shi XQ, Welander U, Angmar-Mansson B (2000) Occlusal caries detection with KaVo DIAGNOdent and radiography: an in vitro comparison. Caries Res 34:151–158CrossRefPubMedGoogle Scholar
  11. 11.
    Pereira AC, Verdonschot EH, Huysmans MC (2001) Caries detection methods: can they aid decision making for invasive sealant treatment. Caries Res 35:83–89CrossRefPubMedGoogle Scholar
  12. 12.
    Lussi A, Hellwig E (2006) Performance of a new laser fluorescence device for the detection of occlusal caries in vitro. J Dent 34:467–471CrossRefPubMedGoogle Scholar
  13. 13.
    Anttonen V, Seppa L, Hausen H (2003) Clinical study of the use of the laser fluorescence device DIAGNOdent for detection of occlusal caries in children. Caries Res 37:17–23CrossRefPubMedGoogle Scholar
  14. 14.
    Heinrich-Weltzien R, Kühnisch J, Oehme T, Ziehe A, Stösser L, García-Godoy F (2003) Comparison of different DIAGNOdent cut-off limits for in vivo detection of occlusal caries. Oper Dent 28:672–680PubMedGoogle Scholar
  15. 15.
    Rocha RO, Ardenghi TM, Oliveira LB, Rodrigues CR, Ciamponi AL (2003) In vivo effectiveness of laser fluorescence compared to visual inspection and radiography for the detection of occlusal caries in primary teeth. Caries Res 37:437–441CrossRefPubMedGoogle Scholar
  16. 16.
    Angnes V, Angnes G, Batisttella M, Grande RH, Loguercio AD, Reis A (2005) Clinical effectiveness of laser fluorescence, visual inspection and radiography in the detection of occlusal caries. Caries Res 39:490–495CrossRefPubMedGoogle Scholar
  17. 17.
    Akarsu S, Koprulu H (2006) In vivo comparison of the efficacy of DIAGNOdent by visual inspection and radiographic diagnostic techniques in the diagnosis of occlusal caries. J Clin Dent 17:53–58PubMedGoogle Scholar
  18. 18.
    Bader JD, Shugars DA (2004) A systematic review of the performance of a laser fluorescence device for detecting caries. J Am Dent Assoc 135:1413–1426CrossRefPubMedGoogle Scholar
  19. 19.
    Lussi A, Reich E (2005) The influence of toothpastes and prophylaxis pastes on fluorescence measurements for caries detection in vitro. Eur J Oral Sci 113:141–144CrossRefPubMedGoogle Scholar
  20. 20.
    Ekstrand KR, Ricketss DNJ, Kidd EAM (1997) Reproducibility and accuracy of three methods for assessment of demineralization depth on the occlusal surface: an in vitro examination. Caries Res 31:224–231CrossRefPubMedGoogle Scholar
  21. 21.
    Ástvaldsdóttir Á, Holbrook WP, Tranaeus S (2004) Consistency of DIAGNOdent instruments for clinical assessment of fissure caries. Acta Odontol Scand 62:193–198CrossRefPubMedGoogle Scholar
  22. 22.
    KaVo (2003) Clinical guidelines. KaVo, Biberach. First German issueGoogle Scholar
  23. 23.
    Pinelli C, Campos Serra M, de Castro Monteiro Loffredo L (2002) Validity and reproducibility of a laser fluorescence system for detecting the activity of white-spot lesions on free smooth surfaces in vivo. Caries Res 36:19–24CrossRefPubMedGoogle Scholar
  24. 24.
    Lussi A, Francescut P (2003) Performance of conventional and new methods for the detection of occlusal caries in deciduous teeth. Caries Res 37:2–7CrossRefPubMedGoogle Scholar
  25. 25.
    Francescut P, Zimmerli B, Lussi A (2006) Influence of different storage methods on laser fluorescence values: a two year-study. Caries Res 40:181–185CrossRefPubMedGoogle Scholar
  26. 26.
    Mendes FM, Hissadomi M, Imparato JC (2004) Effects of drying time and the presence of plaque on the in vitro performance of laser fluorescence in occlusal caries of primary teeth. Caries Res 38:104–108CrossRefPubMedGoogle Scholar
  27. 27.
    White JM, Eakle WE (2000) Rationale and treatment approach in minimally invasive dentistry. J Am Dent Assoc 131:13S–19SCrossRefPubMedGoogle Scholar
  28. 28.
    Last JM (1995) A dictionary of epidemiology. Oxford University Press, OxfordGoogle Scholar
  29. 29.
    Verdonschot EH, Abdo H, Frankenmolen FWA (1999) The in vivo performance of a laser fluorescence device compared to visual inspection in occlusal caries diagnosis (abstract). Caries Res 33:283Google Scholar
  30. 30.
    Huysmans MC, Longbottom C, Hintze H, Verdonschot EH (1998) Surface-specific electrical occlusal caries diagnosis: reproducibility, correlation with histological lesion depth, and tooth type dependence. Caries Res 32:330–336CrossRefPubMedGoogle Scholar
  31. 31.
    Tranaeus S, Lindgren LE, Karlsson L, Angmar-Månsson B (2004) In vivo validity and reliability of IR fluorescence measurements for caries detection and quantification. Swed Dent J 28:173–182PubMedGoogle Scholar
  32. 32.
    Olmez A, Tuna D, Oznurhan F (2006) Clinical evaluation of diagnodent in detection of occlusal caries in children. J Clin Pediatr Dent 30:287–291PubMedGoogle Scholar
  33. 33.
    Krause F, Jepsen S, Braun A (2007) Comparison of two laser fluorescence devices for the detection of occlusal caries in vivo. Eur J Oral Sci 115:252–256CrossRefPubMedGoogle Scholar
  34. 34.
    Alkurt MT, Peker I, Arisu HD, Bala O, Altunkaynak B (2007) In vivo comparison of laser fluorescence measurements with conventional methods for occlusal caries detection. Lasers Med Sci DOI  10.1007/s10103-007-0486-2
  35. 35.
    Costa AM, Bezerra AC, Fucks AB (2007) Assessment of the accuracy of visual examination, bite-wing radiographs and DIAGNOdent on the diagnosis of occlusal caries. Eur Arch Paediatr Dent 8:118–122CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag London Limited 2008

Authors and Affiliations

  • Michele Baffi Diniz
    • 1
  • Jonas de Almeida Rodrigues
    • 1
  • Andréia Bolzan de Paula
    • 2
  • Rita de Cássia Loiola Cordeiro
    • 1
  1. 1.Department of Pediatric Dentistry, Araraquara School of DentistrySão Paulo State University (UNESP)AraraquaraBrazil
  2. 2.Department of Dental Materials, School of Dentistry of PiracicabaState University of Campinas (UNICAMP)PiracicabaBrazil

Personalised recommendations