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Photodynamic inhibition of acetylcholinesterase after two-photon excitation of copper tetrasulfophthalocyanine

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Abstract

Sequential two-photon (2-γ) activated copper tetrasulfophthalocyanine (CuPcS4) was shown capable of inactivating acetylcholinesterase (ACE). ACE activity was measured photometrically by the Ellman method. Simultaneous irradiation of ACE in the presence of CuPcS4 with 514 nm (183 mW/cm2) and 670 nm (86 mW/cm2) continuous wave (CW) light induced a 20–50% increase in enzyme inhibition as compared to one-photon (1-γ) irradiation, using either 514- or 670-nm (CW) light at the same fluences. The enzyme activity was not affected by CuPcS4 or light alone, decreased linearly with the irradiation time, and was shown to be oxygen-dependent. We conclude that the photoinactivation of ACE with sequential 2-γ irradiation involves reactive oxygen species produced by the interaction of the upper excited Tn state of CuPcS4 with molecular oxygen. As CuPcS4 shows little activity as a conventional 1-γ photosensitizer, unwanted side effects such as prolonged skin sensitivity are eliminated rendering 2-γ photodynamic therapy advantageous for the treatment of selected medical applications.

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Acknowledgements

Funding for this research was provided by the Canadian Institutes of Health Research (CIHR, grant no. MOP-37768; J. E. v. L.), the Canadian Institute for Photonics Innovations (D. H.), and by the Intelligent Materials and Systems Institute of the University of Sherbrooke (D. H.).

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  1. Department of Nuclear Medicine and Radiobiology,Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, QC, J1H 5N4, Canada

    Youssef Mir, Daniel Houde & Johan E. van Lier

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  1. Youssef Mir
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  2. Daniel Houde
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  3. Johan E. van Lier
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Correspondence to Daniel Houde.

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Mir, Y., Houde, D. & van Lier, J.E. Photodynamic inhibition of acetylcholinesterase after two-photon excitation of copper tetrasulfophthalocyanine. Lasers Med Sci 23, 19–25 (2008). https://doi.org/10.1007/s10103-007-0446-x

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