Lasers in Medical Science

, Volume 20, Issue 1, pp 21–27 | Cite as

In vivo study of the healing processes that occur in the jaws of rabbits following perforation by an Er,Cr:YSGG laser

Original Article


This study investigates the healing process that takes place in the bone and soft tissue of the maxilla and the mandible after perforation by an Er,Cr:YSGG laser device. The jaws of New Zealand white rabbits were irradiated with an Er,Cr:YSGG laser, forming wounds 0.4 mm in diameter. Irradiation parameters were as follows: repetition rate was 20 pulse/s, pulse duration was 140–200 μs, power was 2 W, exposure time was 10 s, energy density was 80 J/cm2. After sacrifice at 0–56 days post-surgery, gross observations and histological examinations were performed. Effective hemostasis was achieved after Er,Cr:YSGG laser surgery. There was a minimal delay before the healing began. After 56 days all of the bone defects had been completely replaced by new bone. In conclusion, the Er,Cr:YSGG laser allows precise surgical ablation with minimal thermal damage to adjacent tissues in vivo. The overall subsequent healing was favorable. This laser may potentially be used in minor oral surgery.


Er,Cr:YSGG laser Jaw Laser surgery Rabbit Thermal damage Wound healing 


  1. 1.
    Dibartolomeo JR (1981) Argon and CO2 lasers in otolaryngology: which one, when and why? Laryngoscope 26(Suppl):5–6Google Scholar
  2. 2.
    Li ZZ, Reinisch L, Merwe WPV (1992) Bone ablation with Er:YAG laser CO2 laser: study of thermal and acoustic effects. Lasers Surg Med 12:79–85Google Scholar
  3. 3.
    Lewandrowski KU, Lorente C, Schomacker KT, Flotte TJ, Wilkes JW, Deutsch TF (1996) Use of the Er:YAG laser for improved planting in maxillofacial surgery. Lasers Surg Med 19:40–45CrossRefPubMedGoogle Scholar
  4. 4.
    Clayman L, Fuller T, Beckman H (1978) Healing of continuous-wave and rapid superpulsed carbon dioxide laser induced bone defects. J Oral Surg 36:932–937Google Scholar
  5. 5.
    Fisher SE, Frame JW (1984) The effects of the carbon dioxide surgical laser on oral tissues. Br J Oral Maxillofac Surg 22:414–425Google Scholar
  6. 6.
    McDavid VG, Cobb CM, Rapley JW, Glaros AG, Spencer P (2001) Laser irradiation of bone: III. Long-term healing following treatment by CO2 and Nd:YAG lasers. J Periodontol 72:174–182Google Scholar
  7. 7.
    Walsh JT, Deutsh TF (1989) Er:YAG laser ablation of tissue: measurement of ablation rates. Lasers Surg Med 9:327–337Google Scholar
  8. 8.
    Nuss RC, Fabian RL, Sarkar R, Puliafito CA (1988) Infrared laser bone ablation. Lasers Surg Med 8:381–391Google Scholar
  9. 9.
    Pratisto H, Frenz M, Ith M et al (1994) Use of 3 μm laser irradiation in middle ear surgery. Proc SPIE 2323:179–184Google Scholar
  10. 10.
    Wang X, Ishizaki NT, Suzuki N, Kimura Y, Matsumoto K (2002) Morphological changes of bovine mandibular bone irradiated by Er,Cr:YSGG laser: an in vitro study. J Clin Laser Med Surg 20:245–250Google Scholar
  11. 11.
    Stein E, Sedlacek T, Fabian RL, Nishioka NS (1990) Acute and chronic effects of bone ablation with a pulsed holmium laser. Lasers Surg Med 10:384–388Google Scholar
  12. 12.
    Payne JT, Peavy GM, Reinisch L, Van Sickle DC (2001) Cortical bone healing following laser osteotomy using 6.1 μ wavelength. Lasers Surg Med 29:38–43Google Scholar
  13. 13.
    Rizoiu IM, Eversole LR, Kimmel AI (1996) Effects of erbium, chromium: yttrium, scandium, gallium, garnet laser on mucocutanous soft tissues. Oral Surg Oral Med Oral Pathol 82:386–395Google Scholar
  14. 14.
    Shah UK, Poe DS, Rebeiz EF, Perrault DF, Pankratov MM, Shapshay SM (1996) Erbium laser in middle ear surgery: in vitro and in vivo animal study. Laryngoscope 106:418–422Google Scholar
  15. 15.
    Friesen LR, Cobb CM, Rapley JW, Brokman LF, Spencer P (1999) Laser irradiation of bone: II. Healing response following treatment by CO2 and Nd:YAG lasers. J Periodontol 70:75–83PubMedGoogle Scholar
  16. 16.
    Buchelt M, Kutschera HP, Katterschafka T, Kiss H, Lang S, Beer R, Losert U (1994) Erb:YAG and Hol:YAG laser osteotomy: the effect of laser ablation on bone healing. Lasers Surg Med 15:373–381Google Scholar
  17. 17.
    Bayly JG, Kartha VB, Steven WH (1963) The absorption spectrum of liquid phase H2O, and D2O from 0.7 μm to 10 μm. Infrared Phys 14:211–223Google Scholar
  18. 18.
    Doyle DB, Bendit EG, Blout ER (1975) Infrared spectroscopy of collagen and collagen-like polypeptides. Biopolymers 14:937–957Google Scholar
  19. 19.
    Bonner RF, Smith PD, Leon M (1986) Quantification of tissue effect due to pulsed Erb:YAG laser at 2.9 μm with beam delivery in a wet field in zirconium fluoride fibers. SPIE 713:2–5Google Scholar
  20. 20.
    Nelson JS, Orenstein A, Liaw LH, Berns MW (1989) Mid-infrared erbium:YAG laser ablation of bone: the effect of laser osteotomy on bone healing. Lasers Surg Med 9:362–374Google Scholar
  21. 21.
    Eriksson AR, Albrektsson T (1983) Temperature threshold levels for heat-induced bone tissue injury: a vital-microscopic study in the rabbit. J Prosthet Dent 50:101–107PubMedGoogle Scholar
  22. 22.
    Herdman RCD, Charlton A, Hinton AE (1993) An in vitro comparison of the erbium:YAG laser and the carbon dioxide laser in laryngeal surgery. J Laryngol Otol 107:908–911Google Scholar

Copyright information

© Springer-Verlag London Limited 2005

Authors and Affiliations

  • Xiaogu Wang
    • 1
  • Chengfei Zhang
    • 2
  • Koukichi Matsumoto
    • 1
  1. 1.Department of EndodonticsShowa University School of DentistryOhta-ku, TokyoJapan
  2. 2.Department of Special Dental Service, School of StomatologyPeking UniversityBeijingChina

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