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Accurate identification of S. pneumoniae using MALDI-TOF mass spectrometry, still a challenge for clinical laboratories?

  • Eric FarfourEmail author
  • Nicolas Degand
  • Anaëlle Muggeo
  • Patricia Marcelino
  • Marc Vasse
  • Thomas Guillard
Letter to the Editor
  • 1 Downloads

To the Editor

Due to its high reliability and convenience for micro-organism identification, MALDI-TOF mass spectrometry is now implemented in a large number of clinical laboratories. However, the Microflex LT and the Compass software (Bruker Daltonics, Bremen, Germany) lack of performance for the accurate identification of Streptococcus pneumoniae, S. pseudopneumoniae, and other Streptococci within the mitis/oralis group (SMOG). Several reports have proposed to solve this issue by implementing the supplier database with additional spectra [1], performing visual analysis of spectra [2, 3], or using algorithms [1, 4]. In comparison to routine use of MALDI-TOF mass spectrometry, most of these strategies are time-consuming, uneasy to perform, or still insufficiently accurate. Furthermore, most reports include a few number of clinical strains and most often no S. pseudopneumoniae.Moreover, most of the strains are frozen collection strains that do not reflect routine use conditions....

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Service de Biologie CliniqueHôpital FochSuresnesFrance
  2. 2.Laboratoire de bactériologie, Centre Hospitalier Universitaire de NiceHôpital de l’ArchetNiceFrance
  3. 3.Inserm UMR-S 1250 P3Cell, SFR CAP-SantéUniversité de Reims-Champagne-ArdenneReimsFrance
  4. 4.Laboratoire de Bactériologie-Virologie-Hygiène Hospitalière-Parasitologie-Mycologie, CHU ReimsHôpital Robert DebréReimsFrance

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