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Distribution and relevance of Dientamoeba fragilis and Blastocystis species in gastroenteritis: results from a case-control study

  • Maria Dullaert- de BoerEmail author
  • Theo A. Schuurs
  • Marloes Vermeer
  • Gijs J.H.M. Ruijs
  • Adri G. M. van der Zanden
  • Jan F. Weel
  • Lesla E.S. Bruijnesteijn van Coppenraet
Original Article

Abstract

The actual role of Dientamoeba fragilis and Blastocystis in patients with gastrointestinal symptoms is still under debate. A multicenter case-control study was performed in The Netherlands to elucidate the clinical relevance of molecular diagnostics results in gastroenteritis (GE). Samples from this case-control study were used to perform a detailed analysis on the presence of D. fragilis and Blastocystis in relation to gastrointestinal symptoms. In the present study, a real-time PCR for Blastocystis was performed on 1374 case samples and 1026 control samples from the multicenter gastroenteritis case-control study previously tested for D. fragilis. Prevalence of both micro-organisms was highest in children under 20 years of age and lowest in the oldest age group. A significantly lower overall detection of D. fragilis and Blastocystis was found in cases (both 25.8%) as compared to controls (37.6% and 40.0%, respectively). The difference for D. fragilis was statistically significant for subjects above 20 years of age. For Blastocystis, the difference was statistically significant in all age groups, except in children less than 5 years of age. A negative relation between D. fragilis-positive cases and diarrhea was found in this study population. More GE symptoms were reported in cases without D. fragilis or Blastocystis. In the present study, prevalence of both D. fragilis and Blastocystis is lower in cases with gastroenteritic symptoms than in controls. Besides, in cases with D. fragilis or Blastocystis, no association is shown between any of the GE symptoms. Interestingly, this suggests that the presence of these protozoans may be considered characteristic of a healthy intestinal microbiome.

Keywords

Dientamoeba fragilis Blastocystis Gastroenteritis Diarrhea Case-control Multiplexed real-time PCR 

Notes

Acknowledgments

The authors acknowledge Rune Stensvold for the technical information and technical support of the Blastocystis PCR and suggestions for the manuscript, Jaco J Verweij for PCR technical advice and fruitful discussions and suggestions for the manuscript, Roche Diagnostics for financial support of nucleic acid extraction, and Life Technologies for financial support of the PCR reactions. Special thanks to V. Tuchter, A. Stellingwerf, O. Lindenboom, M. Janssen, and J. Wallinga for technical support.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Maria Dullaert- de Boer
    • 1
    Email author
  • Theo A. Schuurs
    • 2
  • Marloes Vermeer
    • 3
  • Gijs J.H.M. Ruijs
    • 4
  • Adri G. M. van der Zanden
    • 1
  • Jan F. Weel
    • 2
  • Lesla E.S. Bruijnesteijn van Coppenraet
    • 4
  1. 1.Laboratory for Medical Microbiology and Public HealthHengeloThe Netherlands
  2. 2.Centre for Infectious Diseases FrieslandIzoreLeeuwardenThe Netherlands
  3. 3.ZGT AcademyZGTAlmeloThe Netherlands
  4. 4.Laboratory for Medical Microbiology and Infectious DiseasesIsalaZwolleThe Netherlands

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