A comparative evaluation of BACT/ALERT FA PLUS and FN PLUS blood culture bottles and BD BACTEC Plus Aerobic and Anaerobic blood culture bottles for antimicrobial neutralization

  • Yousun Chung
  • In-Hee Kim
  • Minje Han
  • Hyun Soo Kim
  • Han-Sung Kim
  • Wonkeun Song
  • Jae-Seok KimEmail author
Original Article


The performance of BACT/ALERT FA and FN PLUS (FA PLUS and FN PLUS) blood culture bottles with the BACT/ALERT VIRTUO (bioMérieux, Inc., Durham, NC) and BD BACTEC Plus Aerobic and Anaerobic (BD Aerobic and BD Anaerobic) blood culture bottles with the BD BACTEC FX (BD Diagnostics, Sparks, MD) for antimicrobial neutralization at peak serum concentration was evaluated. The following antibiotic agents and microbial strains were used: ampicillin, cefepime, cefotaxime, gentamicin, levofloxacin, meropenem, piperacillin-tazobactam, and vancomycin; methicillin-sensitive Staphylococcus aureus, Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Bacteroides fragilis. The detection rate of FA PLUS bottles was 69.1% (259/375) and that of BD Aerobic bottles was 75.5% (283/375) (p = 0.050). In the case of anaerobic culture, the overall detection rate of FN PLUS bottles was 77.0% (231/300) and that of BD Anaerobic bottles was 71.3% (214/300) (p = 0.113). The time to detection (TTD) from aerobic culture was 2.8 h shorter in FA PLUS bottles (12.4 h) compared to BD Aerobic bottles (15.2 h) (p < 0.001). And the TTD from anaerobic culture was 1.6 h shorter in FN PLUS bottles (18.1 h) compared to BD Anaerobic bottles (19.7 h) (p = 0.061). The FA PLUS bottles exhibited a lower detection rate compared to BD Aerobic bottles, while FN PLUS bottles showed a higher detection rate compared to BD Anaerobic bottles. The BACT/ALERT VIRTUO system exhibited shorter TTD compared to the BD BACTEC FX system for both aerobic and anaerobic cultures.


Blood culture Antimicrobial neutralization BACT/ALERT FA PLUS BACT/ALERT FN PLUS BD BACTEC Plus Aerobic BD BACTEC Plus Anaerobic 



This study was supported by bioMérieux, France, and by the National Research Foundation of Korea (NRF-2017M3A9E4-007225).

Supplementary material

10096_2019_3663_MOESM1_ESM.docx (112 kb)
ESM 1 (DOCX 111 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Yousun Chung
    • 1
  • In-Hee Kim
    • 1
  • Minje Han
    • 1
  • Hyun Soo Kim
    • 2
  • Han-Sung Kim
    • 2
  • Wonkeun Song
    • 2
  • Jae-Seok Kim
    • 1
    • 2
    Email author
  1. 1.Department of Laboratory Medicine, Kangdong Sacred Heart HospitalHallym University College of MedicineSeoulSouth Korea
  2. 2.Department of Laboratory MedicineHallym University College of MedicineChuncheonSouth Korea

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