Detection of optrA-positive enterococci clinical isolates in Belgium

  • M. Angeles ArgudínEmail author
  • S. Youzaga
  • M. Dodémont
  • A. Heinrichs
  • S. Roisin
  • A. Deplano
  • C. Nonhoff
  • M. Hallin
Letter to the Editor

Dear Editor,

Linezolid, the first member of the oxazolidinone family, is one of the major antimicrobial agents used for the treatment of vancomycin-resistant enterococcus (VRE) infections. This antibiotic binds to the domain V of the 23S ribosomal RNA (rRNA) of the 50S subunit inhibiting the bacterial protein synthesis. Diverse mechanisms conferring linezolid resistance have been described in enterococci, including point mutations in genes encoding 23S rRNA, mutations in ribosomal proteins L3 and/or L4, or the presence of the plasmid-borne ribosomal methyltransferase gene cfr or its variant cfr(B) [1, 2]. The new variant cfr(C) has only been described in Clostridium [3] and Campylobacter [4]. Recently, new oxazolidinone resistance genes coding for an ABC transporter (optrA) and for an ARE ABC-F family protein (poxtA) have been described. Both genes have been detected in plasmids and/or transposons in enterococci and/or staphylococci of human and animal origin [5, 6, 7, 8, 9]. Most...



We would like to thank the Veterinary and Agrochemical Research Centre (CODA-CERVA, Belgium), the National Reference Centre for Staphylococci and Enterococci of the Institute Robert Koch (Germany), the Nation Food Institute of the Technical University of Denmark (Denmark), the “Centre National de Référence des Staphylocoques” of “Hospices Civils de Lyon” (France) and the University of Florence (Italy) for the supply of positive control strains for this study.

Financial support

This study was supported by internal funding.

Compliance with ethical standards

Conflicts of interest

The authors declare that they have no conflict of interest.

Ethical approval

For this type of study formal consent is not required.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Microbiology, Laboratoire Hospitalier Universitaire de Bruxelles (LHUB-ULB) Site Anderlecht, Hôpital Erasme-Cliniques universitaires de BruxellesUniversité Libre de BruxellesBrusselsBelgium

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