Comparison of three multiplex real-time PCR assays for detection of enteric viruses in patients with diarrhea
In this study, the usability and performance of three commercially available multiplex real-time RT-PCR assays for the detection of major enteric viruses were investigated. In total, 481 fecal specimens were analyzed using the Allplex™ GI Virus Assay, the Rida®Gene Viral Stool Panel I, and the FTD Viral Gastroenteritis. The overall agreement between the assays was 99.9%. Despite convergent analytical performance, differences between the multiplex RT-PCR assays were apparent when considering their suitability for routine diagnostics.
KeywordsGastroenteritis Enteric viruses Diarrhea Molecular diagnostics
Siru Koskinen, Linda Luoma, and Hanna Saari are gratefully acknowledged for their help in sample analyses.
This study was carried out alongside routine diagnostics.
Compliance with ethical standards
Conflict of interest
The author reports no conflict of interests.
This article does not contain any studies with human participants or animals performed by the author. There were no requirements for informed consent.
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