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A new kit to detect Campylobacter species in stool specimens: the Orion GenRead Campylobacter®

  • Alice Buissonnière
  • Lucie Bénéjat
  • Paul Charron
  • Emilie Bessède
  • Philippe Lehours
  • Guillaume Valdenaire
  • Olivier Richer
  • Francis Mégraud
Original Article
  • 79 Downloads

Abstract

Campylobacter enteritis is the most frequent bacterial enteritis including in children. Its diagnosis suffers from the lack of sensitivity and delayed result of culture. Our aim was to test a new PCR-derived method for Campylobacter diagnosis in comparison to a composite reference. Patients presenting to the emergency ward of our hospital with enteric symptoms during the 2016 summer season were included. In addition to culture, an ELISA and an in-house real-time PCR were performed, as well as the new method (Orion GenRead Campylobacter) on all stool specimens. The composite reference used to consider a case positive for Campylobacter was either culture positive and in case of negative culture both the ELISA and real-time PCR positive. One hundred fifty patients were included, 64 being infants or children. There were 29 cases positive by the composite reference, with 19 of the 64 children (29.7%) and 10 of the 86 adults (11.6%). If performed alone, culture would have missed six cases. The Orion GenRead Campylobacter detected all the positives by the composite reference but also 12 cases negative by the composite reference (sensitivity 100%, specificity 90.1%). Given the characteristics of the new method, it can be used as a screening method for Campylobacter detection.

Keywords

Enteritis Diagnosis Culture PCR ELISA 

Notes

Funding information

We acknowledge an unrestricted grant from Orion Diagnosis.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

Ethical approval was not necessary and consent forms were obtained.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.French National Reference Centre for Campylobacters and Helicobacters, Bacteriology LaboratoryCHU BordeauxBordeauxFrance
  2. 2.INSERM U1053 BaRITOnUniversity of BordeauxBordeauxFrance
  3. 3.Emergency DepartmentCHU BordeauxBordeauxFrance
  4. 4.Pediatric Emergency DepartmentCHU BordeauxBordeauxFrance

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