A new kit to detect Campylobacter species in stool specimens: the Orion GenRead Campylobacter®

  • Alice Buissonnière
  • Lucie Bénéjat
  • Paul Charron
  • Emilie Bessède
  • Philippe Lehours
  • Guillaume Valdenaire
  • Olivier Richer
  • Francis MégraudEmail author
Original Article


Campylobacter enteritis is the most frequent bacterial enteritis including in children. Its diagnosis suffers from the lack of sensitivity and delayed result of culture. Our aim was to test a new PCR-derived method for Campylobacter diagnosis in comparison to a composite reference. Patients presenting to the emergency ward of our hospital with enteric symptoms during the 2016 summer season were included. In addition to culture, an ELISA and an in-house real-time PCR were performed, as well as the new method (Orion GenRead Campylobacter) on all stool specimens. The composite reference used to consider a case positive for Campylobacter was either culture positive and in case of negative culture both the ELISA and real-time PCR positive. One hundred fifty patients were included, 64 being infants or children. There were 29 cases positive by the composite reference, with 19 of the 64 children (29.7%) and 10 of the 86 adults (11.6%). If performed alone, culture would have missed six cases. The Orion GenRead Campylobacter detected all the positives by the composite reference but also 12 cases negative by the composite reference (sensitivity 100%, specificity 90.1%). Given the characteristics of the new method, it can be used as a screening method for Campylobacter detection.


Enteritis Diagnosis Culture PCR ELISA 


Funding information

We acknowledge an unrestricted grant from Orion Diagnosis.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

Ethical approval was not necessary and consent forms were obtained.


  1. 1.
    Van Cauteren D, Le Strat Y, Sommen C, Bruyand M, Tourdjman M, Jourdan-Da SN, Couturier E, Fournet N, De Valk H, Desenclos JC (2018) Estimation de la morbidité et de la mortalité liées aux infections d'origine alimentaire en France métropolitaine, 2008–2013. Bull Epidemiol Hebd 1:2–10Google Scholar
  2. 2.
    Van Cauteren D, De Valk H, Sommen C, King LA, Jourdan-Da Silva N, Weill F-X, Le Hello S, Mégraud F, Vaillant V, Desenclos JC (2015) Community incidence of campylobacteriosis and nontyphoidal salmonellosis, France, 2008–2013. Foodborne Pathog Dis 12:664–669CrossRefPubMedGoogle Scholar
  3. 3.
    Bessède E, Delcamp A, Sifré E, Buissonnière A, Mégraud F (2011) New methods for detection of campylobacters in stool samples in comparison to culture. J Clin Microbiol 49:941–944CrossRefPubMedPubMedCentralGoogle Scholar
  4. 4.
    Floch P, Goret J, Bessède E, Lehours P, Mégraud F (2012) Evaluation of the positive predictive value of a rapid immunochromatographic test to detect campylobacter in stools. Gut Pathog 4:17CrossRefPubMedPubMedCentralGoogle Scholar
  5. 5.
    Bessède E, Asselineau J, Perez P, Valdenaire G, Richer O, Lehours P, Mégraud F (2018) Evaluation of the diagnostic accuracy of 2 immunochromatographic tests detecting Campylobacters in stools and their role in Campylobacter infection diagnostic. J Clin Microbiol;56(4). pii: e.01567–17. [Epub ahead of print]Google Scholar
  6. 6.
    Salazar-Lindo E, Sack RB, Chea-Woo E, Kay BA, Piscoya ZA, Leon-Barua R, Yi A (1986) Early treatment with erythromycin of Campylobacter jejuni-associated dysentery in children. J Pediatr 109:355–360CrossRefPubMedGoogle Scholar
  7. 7.
    Hoser MJ, Mansukoski HK, Morrical SW, Eboigbodin KE (2014) Strand Invasion Based Amplification (SIBA®): a novel isothermal DNA amplification technology demonstrating high specificity and sensitivity for a single molecule of target analyte. PLoS One 9:e112656CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    Nylen G, Dunstan F, Palmer SR, Bager F, Cowden J, Feierl L, Galloway Y, Kapperud F, Megraud F, Molbak K, Petersen LR, Ruutu P (2002) The seasonal distribution of campylobacter infection in nine European countries and New Zealand. Epidemiol Infect 128:383–390CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.French National Reference Centre for Campylobacters and Helicobacters, Bacteriology LaboratoryCHU BordeauxBordeauxFrance
  2. 2.INSERM U1053 BaRITOnUniversity of BordeauxBordeauxFrance
  3. 3.Emergency DepartmentCHU BordeauxBordeauxFrance
  4. 4.Pediatric Emergency DepartmentCHU BordeauxBordeauxFrance

Personalised recommendations