Mucosa-associated microbiota signature in colorectal cancer

  • R. Gao
  • C. Kong
  • L. Huang
  • H. Li
  • X. Qu
  • Z. Liu
  • P. Lan
  • J. Wang
  • H. Qin
Original Article

Abstract

The aim of this study was to explore the gut microbiota profiles of colorectal cancer (CRC) patients and to examine the relationship between gut microbiota and other key molecular factors involved in CRC tumorigenesis. In this study, a 16S rDNA sequencing platform was used to identify possible differences in the microbiota signature between CRC and adjacent normal mucosal tissue. Differences in the microbiota composition in different anatomical colorectal tumor sites and their potential association with KRAS mutation were also explored. In this study, the number of Firmicutes and Actinobacteria decreased, while the number of Fusobacteria increased in the gut of CRC patients. In addition, at the genus level, Fusobacterium was identified as the key contributor to CRC tumorigenesis. In addition, a different distribution of gut microbiota in ascending and descending colon cancer samples was observed. Lipopolysaccharide biosynthesis-associated microbial genes were enriched in tumor tissues. Our study suggests that specific mucosa-associated microbiota signature and function are significantly changed in the gut of CRC patients, which may provide insight into the progression of CRC. These findings could also be of value in the creation of new prevention and treatment strategies for this type of cancer.

Notes

Acknowledgments

We thank Majorbio Biological Technology Co., Ltd. for the technical assistance in the study.

Author contributions

RG, CK, and HL wrote the manuscript; RG, CK, and LH collected the clinical data; RG and ZL provided the samples; RG, HL, XQ, and ZL analyzed the data. PL, JW, and HQ designed and supervised the study.

Compliance with ethical standards

Funding

This work was supported by grants from the National Natural Science Foundation of China (nos. 81230057, 81200264, 81372615 and 81472262) and Emerging Cutting-Edge Technology Joint Research projects of Shanghai (no. SHDC12012106). Tongji University Subject Pilot Program (no.162385).

Conflict of interest

No conflict.

Ethical approval

This study has been approved by the ethical committees of Shanghai Tenth People’s Hospital. and the Sixth Affiliated Hospital, Sun Yat-sen University.

Informed consent

All the participants provided informed consents.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  1. 1.Department of GI SurgeryShanghai Tenth People’s Hospital Affiliated to Tongji University School of MedicineShanghaiChina
  2. 2.Research Institute of Intestinal DiseasesTongji University School of MedicineShanghaiChina
  3. 3.Department of GI Surgery, the Sixth Affiliated HospitalSun Yat-sen UniversityGuangdong ProvinceChina

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