Comparison of Xpert Norovirus and RidaGene Norovirus assays for the detection of noroviruses in clinical fecal specimens
The purpose of this study was to investigate the usability and performance of the Xpert Norovirus and RidaGene Norovirus assays for the detection of noroviruses in fecal specimens. Of the 186 stool specimens, 53 (28.5%) were considered true-positive for norovirus (NoV). Of the true-positive specimens, Xpert detected 53 and RidaGene detected 52. The respective sensitivity and specificity were 100% and 94.7% [95% confidence interval (CI), 91.0–98.5%] for the Xpert assay, and 98.1% (95% CI, 94.4–100%) and 97.0% (95% CI, 94.1–99.9%) for the RidaGene assay. Positive and negative predictive values (PPVs and NPVs) were 88.3% and 100% for the Xpert assay, and 92.9% and 99.2% for the RidaGene assay, respectively. Based on this study, it can be concluded that there were no significant differences (p-value > 0.5) between the results of the Xpert and RidaGene Norovirus assays. We found that both assays are useful for the detection of noroviruses in clinical stool samples.
KeywordsReverse Transcription Polymerase Chain Reaction Antigen Test Reverse Transcription Polymerase Chain Reaction Assay Xpert Assay Nucleic Acid Extraction Protocol
The work at Seinäjoki Central Hospital was part of the TUKE 5050 project and funded by TUKE (Research and Development Project of The Hospital District of South Ostrobothnia).
Compliance with ethical standards
Conflict of interest
The authors have no conflict of interest to declare.
This article does not contain any studies with human participants or animals performed by any of the authors. Informed consent was obtained from all individual participants included in the study.
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