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Antimicrobial binding and growth kinetics in BacT/ALERT® FA Plus and BACTEC® Aerobic/F Plus blood culture media

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Abstract

The capacity of absorbent beads in BacT/ALERT® FA Plus and BACTEC® Aerobic/F Plus blood culture bottles to bind and neutralize antibiotics was compared. Binding was established using reverse-phase HPLC, and inactivation was based on the recovery of susceptible test stains from simulated blood cultures. The FA Plus medium demonstrated more rapid and better overall binding kinetics for each drug tested, resulting in significantly better overall recovery rates. Differences in time to detection favored the FA Plus medium for three drug/organism combinations and Aerobic/F Plus for two.

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Authors and Affiliations

  1. bioMérieux, Inc., 100 Rodolphe Street, Durham, NC, 27712, USA

    D. Lovern, B. Katzin, K. Johnson, D. Broadwell, E. Miller, A. Gates, P. Deol, K. Doing & W. M. Dunne Jr

  2. bioMérieux, SA, La Balme Les Grottes, France

    A. van Belkum

  3. bioMérieux, Inc., St. Louis, MO, USA

    C. Marshall & E. Mathias

Authors
  1. D. Lovern
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  2. B. Katzin
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  3. K. Johnson
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  4. D. Broadwell
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  5. E. Miller
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  6. A. Gates
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  7. P. Deol
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  8. K. Doing
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  9. A. van Belkum
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  10. C. Marshall
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  11. E. Mathias
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  12. W. M. Dunne Jr
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Corresponding author

Correspondence to W. M. Dunne Jr.

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Funding

No external funding was obtained for the performance of this study.

Informed consent

Since the protocol was based on seeded cultures, patient consent was not required, and no animal models were used in the performance of this study. Human blood for seeded blood culture studies are obtained from volunteer bioMérieux employees who sign an authorization and associated risk form and are compensated for their donation. All other ethical considerations were adhered to in the performance of this study.

Conflict of interest

All authors are employees of bioMérieux, Inc.

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Lovern, D., Katzin, B., Johnson, K. et al. Antimicrobial binding and growth kinetics in BacT/ALERT® FA Plus and BACTEC® Aerobic/F Plus blood culture media. Eur J Clin Microbiol Infect Dis 35, 2033–2036 (2016). https://doi.org/10.1007/s10096-016-2759-9

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  • DOI: https://doi.org/10.1007/s10096-016-2759-9

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