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Detection of signature volatiles for cariogenic microorganisms

  • M. Hertel
  • R. Preissner
  • B. Gillissen
  • A. M. Schmidt-Westhausen
  • S. Paris
  • S. PreissnerEmail author
Original Article

Abstract

The development of a breath test by the identification of volatile organic compounds (VOCs) emitted by cariogenic bacteria is a promising approach for caries risk assessment and early caries detection. The aim of the present study was to investigate the volatile profiles of three major cariogenic bacteria and to assess whether the obtained signatures were species-specific. Therefore, the headspaces above cultures of Streptococcus mutans, Lactobacillus salivarius and Propionibacterium acidifaciens were analysed after 24 and 48 h of cultivation using gas chromatography and mass spectrometry. A volatile database was queried for the obtained VOC profiles. Sixty-four compounds were detected within the analysed culture headspaces and were absent (36) or at least only present in minor amounts (28) in the control headspace. For S. mutans 18, for L. salivarius three and for P. acidifaciens five compounds were found to be unique signature VOCs. Database matching revealed that the identified signatures of all bacteria were unique. Furthermore, 13 of the 64 detected substances have not been previously reported to be emitted by bacteria or fungi. Specific VOC signatures were found in all the investigated bacteria cultures. The obtained results encourage further research to investigate the transferability to in vivo conditions towards the development of a breath test.

Keywords

Breath Test Streptococcus Mutans Volatile Profile Glycol Ether Cariogenic Bacterium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

The authors would like to thank Mss. Anja and Antje Richter for their most valuable assistance in the laboratory and Janette Nickel for her help with the mVOC database.

Compliance with ethical standards

Conflict of interest

The authors state that they have no affiliations with or involvement in any organisation or entity with any financial interest or non-financial interest in the subject matter or materials discussed in this article.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • M. Hertel
    • 1
  • R. Preissner
    • 2
  • B. Gillissen
    • 3
    • 4
  • A. M. Schmidt-Westhausen
    • 1
  • S. Paris
    • 5
  • S. Preissner
    • 5
    Email author
  1. 1.Department of Oral Medicine, Dental Radiology and Oral SurgeryCharité - Universitätsmedizin BerlinBerlinGermany
  2. 2.Structural Bioinformatics Group, Institute for PhysiologyCharité - Universitätsmedizin BerlinBerlinGermany
  3. 3.German Cancer Consortium (DKTK)HeidelbergGermany
  4. 4.Hematology, Oncology and Tumor ImmunologyCharité - Universitätsmedizin BerlinBerlinGermany
  5. 5.Department of Operative and Preventive DentistryCharité - Universitätsmedizin BerlinBerlinGermany

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