A rural worker infected with a bovine-prevalent genotype of Campylobacter fetus subsp. fetus supports zoonotic transmission and inconsistency of MLST and whole-genome typing

  • G. Iraola
  • L. Betancor
  • L. Calleros
  • P. Gadea
  • G. Algorta
  • S. Galeano
  • P. Muxi
  • G. Greif
  • R. Pérez
Article

Abstract

Whole-genome characterisation in clinical microbiology enables to detect trends in infection dynamics and disease transmission. Here, we report a case of bacteraemia due to Campylobacter fetus subsp. fetus in a rural worker under cancer treatment that was diagnosed with cellulitis; the patient was treated with antibiotics and recovered. The routine typing methods were not able to identify the microorganism causing the infection, so it was further analysed by molecular methods and whole-genome sequencing. The multi-locus sequence typing (MLST) revealed the presence of the bovine-associated ST-4 genotype. Whole-genome comparisons with other C. fetus strains revealed an inconsistent phylogenetic position based on the core genome, discordant with previous ST-4 strains. To the best of our knowledge, this is the first C. fetus subsp. fetus carrying the ST-4 isolated from humans and represents a probable case of zoonotic transmission from cattle.

Keywords

Mantle Cell Lymphoma Bendamustine Core Genome Zoonotic Transmission MLST Scheme 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

This work was partially funded by FOCEM (MERCOSUR Structural Convergence Fund), COF 03/11 and by a CSIC (Comisión Sectorial de Investigación Científica) fellowship to GI.

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • G. Iraola
    • 1
    • 2
  • L. Betancor
    • 3
  • L. Calleros
    • 2
  • P. Gadea
    • 3
  • G. Algorta
    • 3
    • 4
  • S. Galeano
    • 5
  • P. Muxi
    • 5
  • G. Greif
    • 6
  • R. Pérez
    • 2
  1. 1.Unidad de BioinformáticaInstitut Pasteur de MontevideoMontevideoUruguay
  2. 2.Sección Genética Evolutiva, Facultad de CienciasUniversidad de la RepúblicaMontevideoUruguay
  3. 3.Departamento de Bacteriología y Virología, Instituto de Higiene, Facultad de MedicinaUniversidad de la RepúblicaMontevideoUruguay
  4. 4.Laboratorio de MicrobiologíaHospital BritánicoMontevideoUruguay
  5. 5.Unidad de HematologíaHospital BritánicoMontevideoUruguay
  6. 6.Unidad de Biología MolecularInstitut Pasteur de MontevideoMontevideoUruguay

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