French regional surveillance program of carbapenemase-producing Gram-negative bacilli: results from a 2-year period

  • A. Pantel
  • A. Boutet-Dubois
  • H. Jean-Pierre
  • H. Marchandin
  • A. Sotto
  • J.-P. Lavigne
  • on behalf of the CARB-LR group


In February 2011, the CARB-LR group was created as a sentinel laboratory-based surveillance network to control the emergence of carbapenem-resistant Gram-negative bacilli (CR GNB) in a French Southern Region. We report the epidemiological results of a 2-year study. All the Gram-negative bacilli isolates detected in the different labs (hospital and community settings) of a French Southern Region and with reduced susceptibility to ertapenem and/or imipenem were characterised with regard to antibiotic resistance, bla genes content, repetitive sequence-based polymerase chain reaction (rep-PCR) profiles and multilocus sequence typing (MLST). A total of 221 strains were analysed. Acinetobacter baumannii was the most prevalent carbapenemase-producing bacteria, with a majority of OXA-23 producers (n = 37). One isolate co-produced OXA-23 and OXA-58 enzymes. Klebsiella pneumoniae was the most frequent carbapenemase-producing Enterobacteriaceae (CPE) (OXA-48 producer: n = 29, KPC producer: n = 1), followed by Escherichia coli (OXA-48 producer: n = 8, KPC producer: n = 1) and Enterobacter cloacae (OXA-48 producer, n = 1). One isolate of Pseudomonas aeruginosa produced a VIM-1 carbapenemase. A clonal diversity of carbapenemase-producing K. pneumoniae and E. coli was noted with different MLSTs. On the other hand, almost all OXA-23-producing A. baumannii strains belonged to the widespread ST2/international clone II. The link between the detection of CR GNB and a foreign country was less obvious, suggesting the beginning of a local cross-transmission. The number of CR GNB cases in our French Southern Region has sharply increased very recently due to the diffusion of OXA-48 producers.


AmpC Ertapenem Doripenem Plasmidic AmpC 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



INSERM U1047 is supported by INSERM and Université de Montpellier 1.

Conflict of interest

The authors report no conflicting interests.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • A. Pantel
    • 1
    • 2
  • A. Boutet-Dubois
    • 2
    • 3
  • H. Jean-Pierre
    • 4
  • H. Marchandin
    • 4
  • A. Sotto
    • 2
    • 5
  • J.-P. Lavigne
    • 1
    • 2
  • on behalf of the CARB-LR group
    • 1
  1. 1.Service de MicrobiologieCHU CarémeauNîmes Cedex 09France
  2. 2.Institut National de la Santé et de la Recherche Médicale, U1047Université Montpellier 1NîmesFrance
  3. 3.Laboratoire de Biologie PolyvalenteCH Alès-CévennesAlèsFrance
  4. 4.Laboratoire de BactériologieCHU Arnaud de VilleneuveMontpellierFrance
  5. 5.Service des Maladies Infectieuses et TropicalesCHU CarémeauNîmesFrance

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