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Activity of colistin in combination with tigecycline or rifampicin against multidrug-resistant Stenotrophomonas maltophilia

  • J. W. Betts
  • L. M. Phee
  • N. Woodford
  • D. W. WarehamEmail author
Article

Abstract

The antimicrobial treatment of Stenotrophomonas maltophilia infections is complicated by intrinsic multidrug resistance and a lack of reliable susceptibility data. We assessed the activity of colistin (COL), rifampicin (RIF) and tigecycline (TGC) alone and in combination using a range of in vitro susceptibility testing methodologies and a simple invertebrate model of S. maltophilia infection (Galleria mellonella). Synergy [fractional inhibitory concentration indices (FICIs) ≤0.5] between COL and either RIF or TGC was observed against 92 % and 88 % of 25 S. maltophilia isolates, respectively, despite resistance to one or another of the single agents alone. In time–kill assays, COL combined with either RIF or TGC was superior to single agents, but only the COL/RIF regimen was reliably bactericidal. The in vitro findings correlated with treatment outcomes in G. mellonella, with heightened survival observed for larvae treated with COL/RIF or COL/TGC compared with COL, RIF or TGC alone. COL combined with RIF was the most effective combination overall in both in vitro and in vivo (p < 0.05) assays. Given the difficulty in selecting appropriate therapy for S. maltophilia infections, regimens consisting of COL combined with RIF or TGC could be considered for clinical use.

Keywords

Cystic Fibrosis Colistin Polymyxin Tigecycline Stenotrophomonas Maltophilia 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

We would like to gratefully acknowledge Pfizer for the supply of tigecycline.

Funding

No specific funding was available for this study.

Competing interests

All authors declare no conflict of interest.

Ethical approval

Not applicable.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • J. W. Betts
    • 1
  • L. M. Phee
    • 1
    • 2
  • N. Woodford
    • 1
    • 3
  • D. W. Wareham
    • 1
    • 2
    Email author
  1. 1.Antimicrobial Research Group, Centre for Immunology and Infectious Disease, Blizard InstituteQueen Mary, University of LondonLondonUK
  2. 2.Division of Infection, Barts Health NHS TrustLondonUK
  3. 3.Antimicrobial Resistance and Healthcare Associated Infections Reference Unit, Public Health England, Reference Microbiology ServicesLondonUK

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