Discrimination of the Acinetobacter calcoaceticusAcinetobacter baumannii complex species by Fourier transform infrared spectroscopy

  • C. Sousa
  • L. Silva
  • F. Grosso
  • A. Nemec
  • J. Lopes
  • L. Peixe
Article

Abstract

The main goal of this work was to assess the ability of Fourier transform infrared spectroscopy with attenuated total reflectance (FTIR-ATR) to discriminate between the species of the Acinetobacter calcoaceticusAcinetobacter baumannii (Acb) complex, i.e. A. baumannii, A. nosocomialis, A. pittii, A. calcoaceticus, genomic species “Between 1 and 3” and genomic species “Close to 13TU”. A total of 122 clinical isolates of the Acb complex previously identified by rpoB sequencing were studied. FTIR-ATR spectra was analysed by partial least squares discriminant analysis (PLSDA) and the model scores were presented in a dendrogram form. This spectroscopic technique proved to be effective in the discrimination of the Acb complex species, with sensitivities from 90 to 100 %. Moreover, a flowchart aiming to help with species identification was developed and tested with 100 % correct predictions for A. baumannii, A. nosocomialis and A. pittii test isolates. This rapid, low cost and environmentally friendly technique proved to be a reliable alternative for the identification of these closely related Acinetobacter species that share many clinical and epidemiological characteristics and are often difficult to distinguish. Its validation towards application on a routine basis could revolutionise high-throughput bacterial identification.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • C. Sousa
    • 1
  • L. Silva
    • 1
  • F. Grosso
    • 1
  • A. Nemec
    • 2
  • J. Lopes
    • 3
  • L. Peixe
    • 1
  1. 1.REQUIMTE, Laboratório de Microbiologia, Departamento de Ciências Biológicas, Faculdade de FarmáciaUniversidade do PortoPortoPortugal
  2. 2.Laboratory of Bacterial GeneticsNational Institute of Public HealthPragueCzech Republic
  3. 3.REQUIMTE, Laboratório de Química Aplicada, Departamento de Ciências Químicas, Faculdade de FarmáciaUniversidade do PortoPortoPortugal

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