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Evaluation of two matrix-assisted laser desorption ionization–time of flight mass spectrometry systems for identification of viridans group streptococci

  • P. Kärpänoja
  • I. Harju
  • K. Rantakokko-Jalava
  • M. Haanperä
  • H. Sarkkinen
Article

Abstract

In this study, the performances of two matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) systems, MALDI Biotyper (Bruker Daltonics) and VITEK MS (bioMérieux), were evaluated in the identification of viridans group streptococci. Two collections of isolates were tested with both methods. From a panel of type collection strains (n = 54), MALDI Biotyper gave correct species-level identification for 51/54 (94 %) strains and 37/54 (69 %) strains for the VITEK MS in vitro diagnostic (IVD) method. Additionally, a collection of blood cultures isolates which had been characterized earlier with partial sequencing of 16S rRNA (n = 97) was analyzed. MALDI Biotyper classified 89 % and VITEK MS 93 % of these correctly to the group level. Comparison of species-level identification from the blood culture collection was possible for 36 strains. MALDI Biotyper identified 75 % and VITEK MS 97 % of these strains consistently. Among the clinical isolates, MALDI Biotyper misidentified 36 strains as Streptococcus pneumoniae. Nevertheless, our results suggest that the current MALDI-TOF methods are a good alternative for the identification of viridans streptococci and do perform as well as or better than commercial phenotypical methods.

Keywords

Group Streptococcus Viridans Group Streptococcus Blood Culture Isolate Optochin Mitis Group Streptococcus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We thank Piritta Peri, Saija Vahanne, Saana Viertomanner, Laura Juska, and Janne Tarhanen for their skillful technical assistance.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10096_2013_2012_MOESM1_ESM.doc (45 kb)
ESM 1 (DOC 45 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • P. Kärpänoja
    • 1
  • I. Harju
    • 2
  • K. Rantakokko-Jalava
    • 2
  • M. Haanperä
    • 3
  • H. Sarkkinen
    • 1
  1. 1.Department of Clinical MicrobiologyPäijät-Häme Social and Health Care Group, Center of Medical ServicesLahtiFinland
  2. 2.Clinical Microbiology LaboratoryTurku University HospitalTurkuFinland
  3. 3.Antimicrobial Resistance UnitNational Institute for Health and WelfareTurkuFinland

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