Association between IL8 haplotypes and pathogen levels in chronic periodontitis
- 248 Downloads
Chronic periodontitis (CP) is considered to be a multifactorial disease influenced by microbial and genetic factors. The aim of the present study was to investigate whether the genetic susceptibility to CP in individuals with the IL8 ATC/TTC haplotype is associated with subgingival levels of periodontopathogens. Sixty-five individuals, grouped according to the presence (n = 28) or absence (n = 37) of the IL8 haplotype, were evaluated. After clinical periodontal evaluation, each group was subdivided according to the presence (CP) or absence (H) of periodontitis. Four subgingival samples were obtained from CP and two samples per subject from H patients. The levels and proportions of Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola were analyzed using quantitative real-time polymerase chain reaction (q-PCR). No differences were found in the proportion of periodontopathogenic bacteria between groups with the presence or absence of the IL8 haplotype. However, in the CP groups, the levels of periodontopathogens were significantly higher in the individuals without the IL8 haplotype than in the individuals with the IL8 haplotype. These results suggest that periodontal destruction may occur in patients who are considered to be genetically susceptible to CP with a lower microbial challenge because of the presence of the IL8 ATC/TTC haplotype than in patients without this haplotype.
KeywordsChronic Periodontitis Gingival Crevicular Fluid Probe Pocket Depth Clinical Attachment Loss Periodontal Destruction
This study was supported by the Foundation for Research Support of São Paulo State (FAPESP) grants 2003/10424-0, 2009/08773-3, and 2009/11371-4.
Conflict of interest
The authors declare no conflicts of interest with respect to the research, authorship, and/or publication of this article.
- 3.Teles R, Sakellari D, Teles F, Konstantinidis A, Kent R, Socransky S, Haffajee A (2010) Relationships among gingival crevicular fluid biomarkers, clinical parameters of periodontal disease, and the subgingival microbiota. J Periodontol 81(1):89–98. doi: 10.1902/jop.2009.090397 PubMedCrossRefGoogle Scholar
- 7.Trombone AP, Cardoso CR, Repeke CE, Ferreira SB Jr, Martins W Jr, Campanelli AP, Avila-Campos MJ, Trevilatto PC, Silva JS, Garlet GP (2009) Tumor necrosis factor-alpha -308G/A single nucleotide polymorphism and red-complex periodontopathogens are independently associated with increased levels of tumor necrosis factor-alpha in diseased periodontal tissues. J Periodontal Res 44(5):598–608. doi: 10.1111/j.1600-0765.2008.01166.x PubMedCrossRefGoogle Scholar
- 9.Anovazzi G, Kim YJ, Viana AC, Curtis KM, Orrico SR, Cirelli JA, Scarel-Caminaga RM (2010) Polymorphisms and haplotypes in the interleukin-4 gene are associated with chronic periodontitis in a Brazilian population. J Periodontol 81(3):392–402. doi: 10.1902/jop.2009.090392 PubMedCrossRefGoogle Scholar
- 11.Duarte PM, da Rocha M, Sampaio E, Mestnik MJ, Feres M, Figueiredo LC, Bastos MF, Faveri M (2010) Serum levels of cytokines in subjects with generalized chronic and aggressive periodontitis before and after non-surgical periodontal therapy: a pilot study. J Periodontol 81(7):1056–1063. doi: 10.1902/jop.2010.090732 PubMedCrossRefGoogle Scholar
- 14.Gomes SC, Nonnenmacher C, Susin C, Oppermann RV, Mutters R, Marcantonio RA (2008) The effect of a supragingival plaque-control regimen on the subgingival microbiota in smokers and never-smokers: evaluation by real-time polymerase chain reaction. J Periodontol 79(12):2297–2304. doi: 10.1902/jop.2008.070558 PubMedCrossRefGoogle Scholar
- 16.Melcher AH (1976) Postgraduate training and graduate education in dentistry. Dent J 42(12):591–598Google Scholar
- 17.Ausubel FM (1999) Short protocols in molecular biology: a compendium of methods from current protocols in molecular biology, 4th edn. Wiley, New YorkGoogle Scholar
- 22.Mineoka T, Awano S, Rikimaru T, Kurata H, Yoshida A, Ansai T, Takehara T (2008) Site-specific development of periodontal disease is associated with increased levels of Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia in subgingival plaque. J Periodontol 79(4):670–676. doi: 10.1902/jop.2008.070398 PubMedCrossRefGoogle Scholar
- 32.Corbi SC, Anovazzi G, Finoti LS, Kim YJ, Capela MV, Secolin R, Marcaccini AM, Gerlach RF, Orrico SR, Cirelli JA, Scarel-Caminaga RM (2012) Haplotypes of susceptibility to chronic periodontitis in the Interleukin 8 gene do not influence protein level in the gingival crevicular fluid. Arch Oral Biol 57(10):1355–1361. doi: 10.1016/j.archoralbio.2012.07.003 PubMedCrossRefGoogle Scholar
- 37.Matarazzo F, Figueiredo LC, Cruz SE, Faveri M, Feres M (2008) Clinical and microbiological benefits of systemic metronidazole and amoxicillin in the treatment of smokers with chronic periodontitis: a randomized placebo-controlled study. J Clin Periodontol 35(10):885–896. doi: 10.1111/j.1600-051X.2008.01304.x PubMedCrossRefGoogle Scholar
- 38.Mestnik MJ, Feres M, Figueiredo LC, Duarte PM, Lira EA, Faveri M (2010) Short-term benefits of the adjunctive use of metronidazole plus amoxicillin in the microbial profile and in the clinical parameters of subjects with generalized aggressive periodontitis. J Clin Periodontol 37(4):353–365. doi: 10.1111/j.1600-051X.2010.01538.x PubMedCrossRefGoogle Scholar