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Genetic variation among methicillin-resistant Staphylococcus aureus isolates from cancer patients in Saudi Arabia

  • M. A. AlreshidiEmail author
  • A. A. Alsalamah
  • R. A. Hamat
  • V. Neela
  • A. S. Alshrari
  • S. S. Atshan
  • H. H. Alajlan
  • M. Nor Shamsudin
Article

Abstract

One hundred and twenty methicillin-resistant Staphylococcus aureus (MRSA) isolated from cancer and non-cancer patients in Saudi Arabia were investigated for antibiotic resistance, virulence determinants and genotypes. The majority of MRSA isolates from cancer (n = 44, 73.3 %) and non-cancer patients (n = 34, 56.7 %) were multi-resistant to more than four classes of antibiotics. Virulence gene profiling showed that all strains were commonly positive for adhesin genes, except ebps and bbp genes, which were not detected in any isolate. Although the presence of adhesin genes varied slightly among MRSA isolates from cancer and non-cancer patients, these variations were not found to be statistically significant. In contrast, the presence of the toxin genes seb, sec, seg and sei was significantly elevated in MRSA strains isolated from cancer patients. Multilocus sequence typing (MLST) detected six and nine sequence types (STs) among isolates from cancer and non-cancer patients, respectively. Using spa typing, 12 and 25 types were detected, including four new types. The ability of different MRSA clones to become multi-resistant and their ability to acquire different virulence factors may contribute to their success as pathogens in individual groups of patients.

Keywords

Toxin Gene Fusidic Acid SCCmec Type Enterotoxin Gene Adhesin Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Funding sources

This work was supported by grant 91857 from the Research University Grant Scheme (RUGS) of Universiti Putra Malaysia (UPM).

Conflict of interest

The authors declare that they have no conflicts of interest.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • M. A. Alreshidi
    • 1
    Email author
  • A. A. Alsalamah
    • 3
  • R. A. Hamat
    • 1
  • V. Neela
    • 1
  • A. S. Alshrari
    • 1
  • S. S. Atshan
    • 1
  • H. H. Alajlan
    • 4
  • M. Nor Shamsudin
    • 1
    • 2
  1. 1.Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health SciencesUniversiti Putra MalaysiaSerdangMalaysia
  2. 2.Laboratory of Marine Science and Aquaculture, Institute of BioscienceUniversiti Putra MalaysiaSerdangMalaysia
  3. 3.Department of Botany and Microbiology, College of ScienceKing Saud UniversityRiyadhSaudi Arabia
  4. 4.Medical LaboratoryPrince Sultan Military Medical CityRiyadhSaudi Arabia

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