Community-acquired methicillin-resistant Staphylococcus aureus clones circulating in Belgium from 2005 to 2009: changing epidemiology

  • J. Brauner
  • M. Hallin
  • A. Deplano
  • R. De Mendonça
  • C. Nonhoff
  • R. De Ryck
  • S. Roisin
  • M. J. Struelens
  • O. Denis
Article

DOI: 10.1007/s10096-012-1784-6

Cite this article as:
Brauner, J., Hallin, M., Deplano, A. et al. Eur J Clin Microbiol Infect Dis (2013) 32: 613. doi:10.1007/s10096-012-1784-6

Abstract

The present study reports the evolution of the demographic characteristics and the molecular epidemiology of community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) in Belgium from 2005 to 2009. Four hundred and ten CA-MRSA isolates were prospectively collected and screened for the presence of Panton–Valentin leucocidin (PVL) and toxic shock syndrome toxin 1 (TSST-1) encoding genes, while clinical information were recorded. PVL- and TSST-1-positive isolates were genotyped by pulsed-field gel electrophoresis (PFGE). Staphylococcal cassette chromosome mec (SCCmec) type, spa type and multilocus sequence type (MLST) were determined on representative isolates. One hundred and fifty-nine (39 %) isolates were PVL-positive. PVL-positive isolates were significantly more frequently isolated from skin or soft tissue than PVL-negative isolates, causing mainly subcutaneous abscesses and furuncles. Patients with PVL-positive CA-MRSA were significantly younger than patients with PVL-negative CA-MRSA. Eighty-seven percent of the PVL-positive isolates belonged to a limited number (n = 7) of PFGE types belonging to sequence types (ST) ST80, ST8, ST30, ST5, ST152, ST338 and a new ST, a single-locus variant of ST1. A temporal evolution of the distribution of these PFGE types was observed, characterised by (1) the dissemination of the ST8-SCCmecIV arcA-positive (USA300) genotype and (2) a genetic diversification. Forty-seven (11 %) strains were TSST-1-positive, of which 65 % clustered into four PFGE types, all belonging to ST5. The epidemiology of CA-MRSA in Belgium is changing, as the rapid diffusion of the USA300 clone seems to occur, together with a clonal diversification.

Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • J. Brauner
    • 1
  • M. Hallin
    • 1
    • 2
  • A. Deplano
    • 1
  • R. De Mendonça
    • 1
  • C. Nonhoff
    • 1
  • R. De Ryck
    • 1
  • S. Roisin
    • 1
  • M. J. Struelens
    • 1
    • 3
  • O. Denis
    • 1
  1. 1.Laboratoire de Référence MRSA-Staphylocoques, Service de MicrobiologieUniversité Libre de Bruxelles (ULB)—Hôpital ErasmeBrusselsBelgium
  2. 2.Service de MicrobiologieHôpital ErasmeBrusselsBelgium
  3. 3.Microbiology Coordination Section, Resource Management and Coordination UnitEuropean Centre for Disease Prevention and Control (ECDC)StockholmSweden

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