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Varying fitness cost associated with resistance to fluoroquinolones governs clonal dynamic of methicillin-resistant Staphylococcus aureus

  • A. Horváth
  • O. Dobay
  • S. Kardos
  • Á. Ghidán
  • Á. Tóth
  • J. Pászti
  • E. Ungvári
  • P. Horváth
  • K. Nagy
  • S. Zissman
  • M. FüziEmail author
Article

Abstract

The purpose of this study was to investigate the impact of fluoroquinolone resistance on the existence and dynamic of MRSA clones. Resistance to ciprofloxacin was induced in strains of community-acquired (CA) MRSA from various sequence types and the fitness cost suffered by mutant derivatives measured in a propagation assay. In addition, the fitness of fluoroquinolone resistant health care-associated (HA) MRSA isolates from major clones prevalent in Hungary were compared with each other and with those of the CA-MRSA derivatives. The genetic background of fluoroquinolone resistance and fitness cost in CA-MRSA was investigated. The fitness cost observed in the CA-MRSA derivatives proved diverse; the derivatives of the ST30-MRSA-IV strain suffered significantly greater fitness cost than those of the ST8-MRSA-IV and ST80-MRSA-IV isolates. Strains from the New York–Japan (ST5-MRSA-II), South German (ST228-MRSA-I) and EMRSA-15 (ST22-MRSA-IV) HA-MRSA clones proved more viable than CA-MRSA derivatives with similar MIC values to ciprofloxacin and HA-MRSA strains from the Hungarian/Brazilian clone (ST239-MRSA-III). Our strains from the New York–Japan, South-German and EMRSA-15 clones seem to have a competitive edge over the tested CA-MRSA isolates in the health care setting. The greater fitness observed in our New York–Japan and South-German strains could account for the replacement by them of the Hungarian/Brazilian clone in Hungary about ten years ago. Alterations in relevant genes were detected. The Ser80 → Phe mutation in the grlA gene may have seriously compromised viability. Surprisingly silent nucleotide substitutions in the grlB gene seemed to impact fitness in derivatives of the ST30-MRSA-IV isolate.

Keywords

Area Under Curve Fitness Cost Fluoroquinolone Resistance Silent Nucleotide Substitution Japan Strain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

Parts of this material were presented at the 16th International Congress of the Hungarian Society for Microbiology, July 2011 (BOP-9). The study was financially supported by the Hungarian National Scientific Research Fund (OTKA), grant no. PD75660.

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • A. Horváth
    • 1
  • O. Dobay
    • 1
  • S. Kardos
    • 1
  • Á. Ghidán
    • 1
  • Á. Tóth
    • 2
  • J. Pászti
    • 2
  • E. Ungvári
    • 2
  • P. Horváth
    • 1
  • K. Nagy
    • 1
  • S. Zissman
    • 1
  • M. Füzi
    • 1
    Email author
  1. 1.Institute of Medical MicrobiologySemmelweis UniversityBudapestHungary
  2. 2.National Center for EpidemiologyBudapestHungary

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