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Evidence of significant synergism between antibiotics and the antipsychotic, antimicrobial drug flupenthixol

  • L. Jeyaseeli
  • A. Dasgupta
  • S. G. Dastidar
  • J. Molnar
  • L. AmaralEmail author
Article

Abstract

Previously, the antipsychotic, non-antibiotic compound flupenthixol dihydrochloride (Fp) was shown to exhibit distinct in vitro antibacterial activity against Gram-positive and Gram-negative bacteria and to significantly protect Swiss albino mice challenged with a known mouse virulent salmonella. The present study was designed to ascertain whether this drug could efficiently augment the action of an antibiotic or a non-antibiotic when tested in combination. A total of 12 bacterial strains belonging to various genera were selected for this study and were sensitive to the antibiotics penicillin (Pc), ampicillin, chloramphenicol, tetracycline, streptomycin, gentamicin, erythromycin, ciprofloxacin, and to the non-antibiotics methdilazine, triflupromazine, promethazine, and Fp. Pronounced and statistically significant synergism (p < 0.01) was observed when Fp was combined with Pc following the disc diffusion assay system. With the help of the checkerboard method, the fractional inhibitory concentration (FIC) index of this pair was found to be 0.375, confirming synergism. This pair of Fp+ Pc was then subjected to in vivo experiments in mice challenged with Salmonella enterica serovar Typhimurium NCTC 74. Statistical analysis of the mouse protection test suggested that this combination was highly synergistic (p < 0.001, Chi-squared analysis). Fp also revealed augmentation of its antimicrobial property when combined with streptomycin, gentamicin, ciprofloxacin, and the non-antibiotic methdilazine. The results of this study may provide alternatives for the therapy of problematic infections such as those associated with Salmonella spp.

Keywords

Minimum Inhibitory Concentration Lacidipine Test Bacterium Fractional Inhibitory Concentration Disc Diffusion Assay 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgement

L. Amaral was supported by the BCC grant SFRH/BCC/51099/2010 provided by the Fundação para a Ciência e a Tecnologia (FCT) of Portugal and PTDC/SAU-FCF/102807/2008 provided by the UPMM.

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • L. Jeyaseeli
    • 1
  • A. Dasgupta
    • 1
    • 5
  • S. G. Dastidar
    • 2
  • J. Molnar
    • 3
  • L. Amaral
    • 4
    Email author
  1. 1.Division of Microbiology, Department of Pharmaceutical TechnologyJadavpur UniversityKolkataIndia
  2. 2.Department of MicrobiologyHerbicure Healthcare Bio-Herbal Research FoundationPailan, KolkataIndia
  3. 3.Institute of Medical Microbiology and Immunobiology, Faculty of MedicineUniversity of SzegedSzegedHungary
  4. 4.Grupo de Micobactérias, Unidade de Microbiologia Médica, UPMM, Instituto de Higiene e Medicina TropicalUniversidade Nova de LisboaLisboaPortugal
  5. 5.Laboratory of Molecular Biology & ImmunologyNational Institute on AgingBaltimoreUSA

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