Characterisation of uropathogenic Escherichia coli from children with urinary tract infection in different countries
- 474 Downloads
Uropathogenic Escherichia coli (UPEC) carry many virulence factors, including those involved in long-term survival in the urinary tract. However, their prevalence and role among UPEC causing urinary tract infection (UTI) in children is not well studied. To further understand the virulence characteristics of these bacteria, we investigated the prevalence of antibiotic resistance, antigen 43 genes, curli and cellulose among UPEC in children from different countries. Isolates (n = 337) from five countries were tested for antibiotic susceptibility, phylogenetic groups, prevalence of flu, fluA CFT073, fluB CFT073, curli and cellulose. High prevalence of multidrug resistance and extended spectrum beta lactamase production was found among Iranian and Vietnamese isolates. Resistance was associated with phylogenetic group D while group B2 was associated with fluA CFT073 and fluB CFT073. Fewer Iranian isolates carried fluA CFT073, curli and cellulose. fluB CFT073 was most prevalent among Slovak isolates. Ampicillin and amoxicillin/clavulanic acid resistance was prevalent among fluA CFT073- and fluB CFT073-positive Australian, Iranian and Swedish isolates. Lack of curli and cellulose was associated with resistance among Vietnamese isolates. We conclude that major differences exist in the prevalence of antibiotic resistance among UPEC from different countries. Associations observed between resistance and virulence factors may, in different ways, promote the long-term survival of UPEC in the urinary tract.
KeywordsUrinary Tract Infection Antibiotic Resistance Phylogenetic Group Recurrent Urinary Tract Infection Ertapenem
Nubia L. Ramos was supported by an Australian Postgraduate Award through the University of the Sunshine Coast, Queensland, Australia. Konrad Stopsack held an MD Fellowship of the Boehringer Ingelheim Fonds. This work was also supported by grants from the Swedish Research Council (56X-20356), ALF Project Funding, Grants from the Swedish International Development Cooperation Agency and Karolinska Institutet.
The authors would like to thank Dr László Kovács from the Department of Pediatrics and Dr Ján Koreň from the Department of Microbiology at Comenius University Medical School, Bratislava, and Dr Malieh Talebi from the Department of Microbiology, University of Iran, Tehran, for the collection of isolates in Slovakia and Iran, respectively. We thank Agneta Hilding from the Department of Molecular Medicine, Karolinska Institutet for assistance with statistical analyses.
- 12.Kai-Larsen Y, Lüthje P, Chromek M, Peters V, Wang X, Holm Å, Kadas L, Hedlund K-J, Johansson J, Chapman MR, Jacobson SH, Römling U, Agerberth B, Brauner A (2010) Uropathogenic Escherichia coli modulates immune responses and its curli fimbriae interact with the antimicrobial peptide LL-37. PLoS Pathog 6:e1001010PubMedCrossRefGoogle Scholar
- 24.Bukh AS, Schønheyder HC, Emmersen JMG, Søgaard M, Bastholm S, Roslev P (2009) Escherichia coli phylogenetic groups are associated with site of infection and level of antibiotic resistance in community-acquired bacteraemia: a 10 year population-based study in Denmark. J Antimicrob Chemother 64:163–168PubMedCrossRefGoogle Scholar
- 25.Johnson JR, Kuskowski MA, Gajewski A, Sahm DF, Karlowsky JA (2004) Virulence characteristics and phylogenetic background of multidrug-resistant and antimicrobial-susceptible clinical isolates of Escherichia coli from across the United States, 2000–2001. J Infect Dis 190:1739–1744PubMedCrossRefGoogle Scholar
- 28.Allsopp LP, Totsika M, Tree JJ, Ulett GC, Mabbett AN, Wells TJ, Kobe B, Beatson SA, Schembri MA (2010) UpaH is a newly identified autotransporter protein that contributes to biofilm formation and bladder colonization by uropathogenic Escherichia coli CFT073. Infect Immun 78(4):1659–1669PubMedCrossRefGoogle Scholar