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Glycopeptide resistance in coagulase-negative staphylococci isolated in blood cultures from patients with hematological malignancies during three decades

  • E. AhlstrandEmail author
  • K. Svensson
  • L. Persson
  • U. Tidefelt
  • B. Söderquist
Article

Abstract

The aim of this study was to determine if there was a long-term increase in glycopeptide minimum inhibitory concentration (MIC) values, MIC creep, among bloodstream isolates of Staphylococcus epidermidis and S. haemolyticus isolated from patients with hematological malignancies. We conducted a retrospective single-center study where all positive blood cultures of S. epidermidis (n = 387) and S. haemolyticus (n = 19) isolated from patients with hematological malignancies during three decades, 1980 to 2009, were re-evaluated for the presence of reduced susceptibility to vancomycin and teicoplanin. Three different methods for the detection of reduced susceptibility to glycopeptides were used; standard Etest, macromethod Etest, and glycopeptide resistance detection (GRD) Etest. The median MIC value for vancomycin was 2 mg/L. MIC values for vancomycin and teicoplanin did not show any statistically significant increase during the study period. The presence of heterogeneously glycopeptide-intermediate staphylococci (hGIS) was analyzed among 405 coagulase-negative staphylococci (CoNS) isolates. hGIS were found in 31–45% of the CoNS isolates by the macromethod Etest and in 53–67% by the GRD Etest during the three decades. In conclusion, we did not observe any long-term glycopeptide MIC creep determined by the standard Etest, although a high and increasing proportion of heterogeneous vancomycin resistance was observed.

Keywords

Minimum Inhibitory Concentration Vancomycin Hematological Malignancy Glycopeptide Teicoplanin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The study was supported by grants from the research committee of Örebro County Council, Sweden.

Transparency declarations

B. Söderquist has been a consultant for Pfizer and Janssen-Cilag.

Funding

Research Committee of Örebro County Council, Sweden.

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • E. Ahlstrand
    • 1
    • 4
    Email author
  • K. Svensson
    • 2
  • L. Persson
    • 3
  • U. Tidefelt
    • 1
    • 4
  • B. Söderquist
    • 2
    • 3
    • 4
  1. 1.Department of Medicine, Division of HematologyÖrebro University HospitalÖrebroSweden
  2. 2.Department of Laboratory Medicine, Clinical MicrobiologyÖrebro University HospitalÖrebroSweden
  3. 3.Department of Infectious DiseasesÖrebro University HospitalÖrebroSweden
  4. 4.School of Health and Medical SciencesÖrebro UniversityÖrebroSweden

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