Clinical evaluation of the Copan ESwab for methicillin-resistant Staphylococcus aureus detection and culture of wounds
- 447 Downloads
The screening for and diagnosis of bacteriological infections often involves the collection and transportation of swab samples. The Copan ESwab was compared with the dry cotton Copan swab for methicillin-resistant Staphylococcus aureus (MRSA) screening (n = 200 paired samples) and with the Amies agar gel swab (Copan) for the sampling of burn and orthopaedic wounds (n = 203 paired samples) in terms of Gram staining and bacterial recovery. Gram stains performed with ESwab liquid showed significantly more Gram-negative rods, streptococci, Gram-positive cocci, Gram-positive rods, polymorphonuclear cells, lymphocytes and red blood cells than Gram stains from dry swabs. Bacterial recovery was significantly higher with ESwab (p < 0.01, for both MRSA screening and wounds, quantitative/semi-quantitative method). This lead to a slightly higher detection rate of MRSA (128 vs. 124 MRSA-positive ESwabs and dry swabs, respectively, p = 0.50) and a higher detection rate of coagulase-negative Staphylococcus spp. (44 isolates with ESwab vs. 29 with Amies gel swab, p = 0.001) and Enterococcus spp. (15 isolates with ESwab vs. 7 isolates with Amies gel swab, p = 0.005) with ESwab (quantitative method). We confirmed that ESwab has a high performance for Gram stains and a higher bacterial recovery than dry and Amies gel swabs when using clinical samples for MRSA screening and wound sampling.
KeywordsMannitol Salt Agar Bacterial Recovery Bacterial Morphotypes Maldi BioTyper Rayon Swab
We sincerely thank Rudi Deleus (Intensive Care Unit Burn Wounds), Godelieve Nicaise, Raf Beneens and Bart Vander Elst (Geriatric Medicine), Marleen Van Eygen (General Internal Medicine) and Jan Bosque (Septic Orthopaedics) for their readiness to procure all of the clinical samples, and Jos Vandeven (Laboratory Medicine) for the help in the identification of the micro-organisms.
Both the ESwab MRSA Collection Kits and the conventional ESwabs were kindly provided by Copan.
- 3.Copan Innovation (2008) ESwab collection and transport system. Product insert and how to use guide. Copan Innovation, Brescia, ItalyGoogle Scholar
- 6.Body B, Arbique J, Bourbeau P et al (2003) Quality Control of Microbiological Transport Systems; Clinical Laboratory and Standards Institute (CLSI) Approved Standard M40-A. Pennsylvania, PA, USA. Dec. report no.: M40-A, volume 23, number 34Google Scholar
- 12.Human RP, Jones GA (2006) A new concept for transporting clinical material on flocked swabs in liquid Amies medium. In: Proceedings of the 106th General Meeting of the American Society for Microbiology (ASM), Orlando, FL, USA, 21–25 May 2006Google Scholar