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Multiplex PCR to determine Streptococcus pneumoniae serotypes causing otitis media in the Republic of Ireland with further characterisation of antimicrobial susceptibilities and genotypes

  • I. VickersEmail author
  • D. O’Flanagan
  • M. Cafferkey
  • H. Humphreys
Article

Abstract

The purpose of this study was to determine the serotypes, genotypes and antimicrobial susceptibilities of Streptococcus pneumoniae causing otitis media (OM) in children in Dublin, Ireland. S. pneumoniae isolates (n = 28) from spontaneously discharging OM were studied. Serotyping was performed using a previously undescribed multiplex polymerase chain reaction (PCR) scheme in combination with serological methods. Multilocus sequence typing (MLST) was performed using standard procedures. Antimicrobial susceptibility testing was performed using the Etest method. Fourteen different S. pneumoniae serotypes were identified. The five most common serotypes were 3, 19F, 19A, 14 and 6A, which accounted for 68% of all infections. The 7-valent pneumococcal conjugate vaccine (PCV7), 10-valent pneumococcal conjugate vaccine (PHiD-CV) and 13-valent pneumococcal conjugate vaccine (PCV13) provided potential coverages of 43%, 46% and 86%, respectively. Reduced susceptibility to penicillin was evident for 25% of isolates and was associated with serotypes 14, 19A, 19F and 9V. A total of 21 different sequence types (STs) were identified. Pneumococcal Molecular Epidemiology Network (PMEN) clones or their variants represented 54% (15/28) of all isolates. Continued monitoring and characterisation of S. pneumoniae causing OM in Ireland is warranted in order to guide future vaccine and treatment policies.

Keywords

Otitis Medium Multiplex Polymerase Chain Reaction Tigecycline Pneumococcal Conjugate Vaccine Penicillin Resistance 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

We are grateful to the staff of the Children’s University Hospital, Temple St., Dublin, for their assistance with the collection of the isolates used in this study. We also wish to thank the Health Protection Surveillance Centre, which financially supports the laboratory surveillance of pneumococcal disease in Ireland. We acknowledge the use of the pneumococcal MLST database, which is located at Imperial College London and funded by the Wellcome Trust.

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • I. Vickers
    • 1
    • 2
    Email author
  • D. O’Flanagan
    • 3
  • M. Cafferkey
    • 1
    • 2
  • H. Humphreys
    • 2
    • 4
  1. 1.Epidemiology and Molecular Biology Unit and Irish Meningococcal and Meningitis Reference LaboratoryChildren’s University HospitalDublinIreland
  2. 2.Department of Clinical Microbiology, RCSI Education and Research CentreBeaumont HospitalDublinIreland
  3. 3.Health Protection Surveillance CentreDublinIreland
  4. 4.Department of MicrobiologyBeaumont HospitalDublinIreland

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