Utility of aptamer-fluorescence in situ hybridization for rapid detection of Pseudomonas aeruginosa
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Pseudomonas aeruginosa, a ubiquitous Gram-negative bacteriuma, is considered one of the most important causes of nosocomial infections in immunocompromised patients with cancer, transplantation, burn or cystic fibrosis [1, 2, 3, 4]. Rapid and accurate identification of P. aeruginosa has important implications for the therapy and management of infectious diseases caused by P. aeruginosa. Numerous new methods such as immunoassays and molecular techniques have been developed for its detection. However, these techniques usually go through several steps including isolation, enrichment and/or purification and require sophisticated equipment and highly trained personnel, which increase the assay time and cost [5, 6]. As a result, culture remains the gold standard. The requirement for a potent new method to identify P. aeruginosa accurately, rapidly and simply is obvious and compelling.
It is well established that fluorescence in situ hybridization (FISH) is a highly valuable tool...
KeywordsAcinetobacter Baumannii Peptide Nucleic Acid Probe Bright Green Fluorescence Aptamer Probe Selection Buffer
This work was supported by Key Program (No. 06G038) from the Military Medical Science and Technique Foundation during the 11th Five-Year Plan Period and the Natural Science Foundation of Fujian Province (No. 2010J05080).