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Molecular typing of Mycobacterium tuberculosis circulated in Moscow, Russian Federation

  • M. V. Afanas’evEmail author
  • L. N. Ikryannikova
  • E. N. Il’ina
  • A. V. Kuz’min
  • E. E. Larionova
  • T. G. Smirnova
  • L. N. Chernousova
  • V. M. Govorun
Article

Abstract

The present study investigates epidemiological diversity and multidrug resistance spreading among Mycobacterium tuberculosis strains circulating in Moscow, Russian Federation. Among 115 M. tuberculosis strains selected randomly from the sputum of epidemiologically unrelated tuberculosis (TB) patients, multidrug-resistant (MDR) strains predominated. Mutations in the RRDR of the rpoB gene were detected in 64 (83.1%) of 77 rifampicin (RIF)-resistant strains. The Ser531→Leu substitution was prevalent among them (76.5%). Aberrations in the Ser315 codon of katG and/or in the inhA promoter region were found in 79 (84.0%) of 94 isoniazid (INH)-resistant strains. Strains belonging to the Beijing family prevailed. Seventy-one different patterns were identified using the 24-VNTR loci typing scheme. Three main 24-loci VNTR clusters included 34 strains which belonged to the Beijing family. The spoligotyping and 24-loci VNTR typing combination demonstrated maximal discriminatory power. Among the Beijing strains, the MDR phenotype was revealed more frequently than among the others. High genetic heterogeneity of the studied population was shown by the assessment of VNTR loci variability in the analyzed group and in the strains from other parts of Russia. Comparison of the 24-VNTR locus typing and spoligotyping data with revealed resistance-associated mutation allows us to make a suggestion that the active transmission of MDR strains and the independent appearance of drug resistance during chemotherapy occurred in the studied population simultaneously.

Keywords

rpoB Gene Tuberculosis Strain Beijing Strain katG Gene Beijing Family 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We are grateful to V.A. Karpov for the oligonucleotide primers synthesis.

Supplementary material

10096_2010_1067_MOESM1_ESM.xls (68 kb)
Supplementary Table 1 (XLS 67 kb)

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • M. V. Afanas’ev
    • 1
    Email author
  • L. N. Ikryannikova
    • 1
  • E. N. Il’ina
    • 1
  • A. V. Kuz’min
    • 2
  • E. E. Larionova
    • 2
  • T. G. Smirnova
    • 2
  • L. N. Chernousova
    • 2
  • V. M. Govorun
    • 1
  1. 1.Research Institute of Physical-Chemical Medicine of the Ministry of Public Health of the Russian FederationMoscowRussia
  2. 2.Central Tuberculosis Research InstituteRussian Academy of Medical SciencesMoscowRussia

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