Comparative study of serology and histology based detection of Helicobacter pylori infections: a large population-based study of 7,241 subjects from China

  • Y. H. Gong
  • L. P. Sun
  • S. G. Jin
  • Y. YuanEmail author
Brief Report


Two detection methods for H. pylori infections, i.e. serological antibody titer measurements by Hp-ELISA and histological scoring by HE staining, have been compared to investigate the relationship between the diagnosis methods, to clarify the application scope of each diagnosis method and to determine its influencing factors. In the 7,241 subjects who participated in gastric cancer screening, H. pylori infection rate was 70.14% by the Hp-HE method and 41.87% by the Hp-ELISA method when 34EIU was recognized as the cut-off value. The IgG titers increased with the elevation of HE scores; however, the two methods were not closely correlated among those in different gastric disease status. Age, gender and drinking status did not have significant impact on the relationship between the two methods; however, smoking status seemed to significantly influence the correlation of the two diagnosis methods. In conclusion, it was necessary to reevaluate the cut-off value when using ELISA test kits in different population groups. In most cases, the results of two H. pylori infection diagnosis methods show high correlation. However, this relationship can be affected by smoking and gastric diseases status. Additionally, the dynamic change of H. pylori antibody titers is an indicator of gastric disease development.


Gastric Cancer Antibody Titer Pylorus Infection Atrophic Gastritis Diagnosis Method 
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All authors declare no conflict of interest in relation to this study. This work was supported by grants from China National Tenth Five-year Study Program for Taking Key Scientific Problems (No. 2004BA703B06-2), the Foundation of the Key Technologies Program in Liaoning Province (No.20072255001-1) and the Foundation of the Key Laboratory of Cancer Intervention in Liaoning Province (No. 2009 S106).


  1. 1.
    Marchildon PA, Ciota LM et al (1996) Evaluation of three commercial enzyme immunoassays compared with the 13C urea breath test for detection of Helicobacter pylori infection. J Clin Microbiol 34(5):1147–1152PubMedGoogle Scholar
  2. 2.
    Leung WK, Ng EK et al (1999) Evaluation of three commercial enzyme-linked immunosorbent assay kits for diagnosis of Helicobacter pylori in Chinese patients. Diagn Microbiol Infect Dis 34(1):13–17CrossRefPubMedGoogle Scholar
  3. 3.
    de Angelis GL, Cavallaro LG et al (2007) Usefulness of a serological panel test in the assessment of gastritis in symptomatic children. Dig Dis 25(3):206–213CrossRefPubMedGoogle Scholar
  4. 4.
    Kreuning J, Lindeman J, Biemond I, Lamers CB (1994) Relation between IgG and IgA antibody titres against Helicobacter pylori in serum and severity of gastritis in asymptomatic subjects. J Clin Pathol 47:227–231CrossRefPubMedGoogle Scholar
  5. 5.
    Hsu PI, Lai KH et al (1997) Correlation of serum immunoglobulin G Helicobacter pylori antibody titers with histologic and endoscopic findings in patients with dyspepsia. J Clin Gastroenterol 25(4):587–591CrossRefPubMedGoogle Scholar
  6. 6.
    Koivisto TT, Voutilainen ME et al (2008) Effect of smoking on gastric histology in Helicobacter pylori-positive gastritis. Scand J Gastroenterol 43(10):1177–1183CrossRefPubMedGoogle Scholar
  7. 7.
    Loffeld RJ, Werdmuller BF et al (2000) IgG antibody titer against Helicobacter pylori correlates with presence of cytotoxin associated gene A-positive H. pylori strains. FEMS Immunol Med Microbiol 28(2):139–141CrossRefPubMedGoogle Scholar
  8. 8.
    Chen TS, Li FY et al (2002) Immunoglobulin G antibody against Helicobacter pylori: clinical implications of levels found in serum. Clin Diagn Lab Immunol 9(5):1044–1048PubMedGoogle Scholar
  9. 9.
    Sharma TK, Prasad VM et al (1996) Quantitative noninvasive testing for Helicobacter pylori does not predict gastroduodenal ulcer disease. Gastrointest Endosc 44(6):679–682CrossRefPubMedGoogle Scholar
  10. 10.
    Fujioka N, Fahey MT et al (2001) Serological Immunoglobulin G antibody titers to Helicobacter pylori in Japanese Brazilian and non-Japanese Brazilian gastric cancer patients and controls in Sao Paulo. Jpn J Cancer Res 92(8):829–835PubMedGoogle Scholar

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  1. 1.Cancer Control Laboratory of Cancer Institute and General SurgeryThe First Affiliated Hospital of China Medical University, the Key Laboratory of Cancer Control in Liaoning ProvinceShenyangChina
  2. 2.Department of Molecular Genetics and MicrobiologyUniversity of FloridaGainesvilleUSA

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