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Emergence of a colistin-resistant KPC-2-producing Klebsiella pneumoniae ST258 clone in Hungary

  • Á. Tóth
  • I. Damjanova
  • E. Puskás
  • L. Jánvári
  • M. Farkas
  • A. Dobák
  • K. Böröcz
  • J. Pászti
Article

Abstract

Nine Klebsiella pneumoniae isolates showing non-susceptibility to carbapenems were collected from three centres in the north-eastern region of Hungary. The minimum inhibitory concentrations (MICs) of antibiotics were determined by Etest. The putative production of a carbapenemase was tested by the modified Hodge test. The presence of bla KPC genes was verified by polymerase chain reaction (PCR) and sequencing. Furthermore, molecular typing was performed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). All isolates showed extensively drug-resistant (XDR) phenotype, and of these, eight isolates were highly resistant to colistin. The isolates carried bla KPC-2, bla SHV-12, bla TEM-1 and bla SHV-11. PFGE analysis of the nine KPC-2-producing Hungarian ST258 K. pneumoniae isolates, two KPC-2-producing Norwegian ST258 isolates and 33 CTX-M-15-producing ST11 isolates revealed the existence of one genetic cluster at an 88% similarity level. The overall results of the PFGE clustering, MLST and the presence of SHV-11 in both ST11 and ST258 suggest that this is the first hyperepidemic clonal complex of multidrug-resistant K. pneumoniae, probably CC258/CC340, possibly undergoing worldwide spread.

Keywords

Colistin Ertapenem Dipicolinic Acid Klebsiella Pneumoniae Carbapenemases Epidemic Clone 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

We are grateful to Ørjan Samuelsen for providing the K47-25 and K48-58 KPC-2-producing Klebsiella pneumoniae strains, to Christian G. Giske for his critical reading of the manuscript and to Mrs. János Topf for her skilful technical assistance.

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Á. Tóth
    • 1
  • I. Damjanova
    • 2
  • E. Puskás
    • 3
  • L. Jánvári
    • 1
  • M. Farkas
    • 4
  • A. Dobák
    • 4
  • K. Böröcz
    • 5
  • J. Pászti
    • 2
  1. 1.Department of BacteriologyNational Center for EpidemiologyBudapestHungary
  2. 2.Department of Phage Typing and Molecular EpidemiologyNational Center for EpidemiologyBudapestHungary
  3. 3.Laboratory of MicrobiologyNorthern Regional Institution of Hungarian National Public Health and Medical Officer ServiceMiskolcHungary
  4. 4.Laboratory of MicrobiologyCorden International Ltd.BudapestHungary
  5. 5.Department of Hospital Epidemiology and HygieneNational Center for EpidemiologyBudapestHungary

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