Advertisement

First human isolates of methicillin-resistant Staphylococcus aureus sequence type 398 in Spain

  • C. PotelEmail author
  • M. Álvarez-Fernández
  • L. Constenla
  • P. Álvarez
  • S. Perez
Brief Report

To the Editor,

An emerging sequence type (ST) 398 methicillin-resistant Staphylococcus aureus (MRSA) clone producing infections in humans has been detected across Europe [1]. Recent studies indicate that it is widely distributed in farm animals, particularly in pigs, suggesting transmission between animals and humans [2].

The frequency of MRSA ST398 in the Spanish population is unknown. In this study, we present the first three human cases of MRSA ST398 infections admitted to two hospitals in the north-west of Spain.

Forty-four MRSA strains were isolated in 2006 in both hospitals. They were studied by the analysis of restriction fragment length polymorphism (RFLP) of the coagulase gene patterns and pulsed-field gel electrophoresis (PFGE) [3, 4]. Three non-Sma I typeable MRSA strains were identified; the three strains were EagI PFGE typeable. Next, they were analyzed by multilocus sequence typing (MLST) and spa typing [5, 6]. The staphylococcal chromosome cassette (SCC) mec, the...

Keywords

Levofloxacin Tobramycin Multilocus Sequence Typing PFGE Typeable ST398 Isolate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was supported by grants from Fondo de Investigaciones Sanitarias (07/0812) and Consellería de Sanidade (PS08/34), Spain.

References

  1. 1.
    Wulf M, Voss A (2008) MRSA in livestock animals—an epidemic waiting to happen? Clin Microbiol Infect 14:519–521CrossRefPubMedGoogle Scholar
  2. 2.
    Smith TC, Male MJ, Harper AL, Kroeger JS, Tinkler GP, Moritz ED, Capuano AW, Herwaldt LA, Diekema DJ (2009) Methicillin-resistant Staphylococcus aureus (MRSA) strain ST398 is present in midwestern U.S. swine and swine workers. PLoS ONE 4(1):e4258CrossRefPubMedGoogle Scholar
  3. 3.
    Potel C, Álvarez M, Álvarez P, Otero I, Fluiters E (2007) Evolution, antimicrobial susceptibility and assignment to international clones of methicillin-resistant Staphylococcus aureus isolated over a 9-year period in two Spanish hospitals. Clin Microbiol Infect 13:728–730CrossRefPubMedGoogle Scholar
  4. 4.
    Murchan S, Kaufmann ME, Deplano A, de Ryck R, Struelens M, Zinn CE, Fussing V, Salmenlinna S, Vuopio-Varkila J, El Solh N, Cuny C, Witte W, Tassios PT, Legakis N, van Leeuwen W, van Belkum A, Vindel A, Laconcha I, Garaizar J, Haeggman S, Olsson-Liljequist B, Ransjo U, Coombes G, Cookson B (2003) Harmonization of pulsed-field gel electrophoresis protocols for epidemiological typing of strains of methicillin-resistant Staphylococcus aureus: a single approach developed by consensus in 10 European laboratories and its application for tracing the spread of related strains. J Clin Microbiol 41:1574–1585CrossRefPubMedGoogle Scholar
  5. 5.
    Enright MC, Day NP, Davies CE, Peacock SJ, Spratt BG (2000) Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus. J Clin Microbiol 38(3):1008–1015PubMedGoogle Scholar
  6. 6.
    Harmsen D, Claus H, Witte W, Rothgänger J, Claus H, Turnwald D, Vogel U (2003) Typing of methicillin-resistant Staphylococcus aureus in a university hospital setting by using novel software for spa repeat determination and database management. J Clin Microbiol 41:5442–5448CrossRefPubMedGoogle Scholar
  7. 7.
    Zhang K, McClure JA, Elsayed S, Louie T, Conly JM (2005) Novel multiplex PCR assay for characterization and concomitant subtyping of staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus. J Clin Microbiol 43:5026–5033CrossRefPubMedGoogle Scholar
  8. 8.
    Shopsin B, Mathema B, Alcabes P, Said-Salim B, Lina G, Matsuka A, Martinez J, Kreiswirth BN (2003) Prevalence of agr specificity groups among Staphylococcus aureus strains colonizing children and their guardians. J Clin Microbiol 41:456–459CrossRefPubMedGoogle Scholar
  9. 9.
    Lina G, Piémont Y, Godail-Gamot F, Bes M, Peter MO, Gauduchon V, Vandenesch F, Etienne J (1999) Involvement of Panton–Valentine leukocidin-producing Staphylococcus aureus in primary skin infections and pneumonia. Clin Infect Dis 29:1128–1132CrossRefPubMedGoogle Scholar
  10. 10.
    Tenover FC, Arbeit RD, Goering RV, Mickelsen PA, Murray BE, Persing DH, Swaminathan B (1995) Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 33(9):2233–2239PubMedGoogle Scholar
  11. 11.
    van Rijen MM, van Keulen PH, Kluytmans JA (2008) Increase in a Dutch hospital of methicillin-resistant Staphylococcus aureus related to animal farming. Clin Infect Dis 46:261–263CrossRefPubMedGoogle Scholar
  12. 12.
    Lewis HC, Mølbak K, Reese C, Aarestrup FM, Selchau M, Sørum M, Skov RL (2008) Pigs as source of methicillin-resistant Staphylococcus aureus CC398 infections in humans, Denmark. Emerg Infect Dis 14:1383–1389CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • C. Potel
    • 1
    Email author
  • M. Álvarez-Fernández
    • 2
  • L. Constenla
    • 3
  • P. Álvarez
    • 4
  • S. Perez
    • 1
  1. 1.Microbiology LaboratoryComplejo Hospitalario Universitario de VigoPontevedraSpain
  2. 2.Microbiology Laboratory, Research LaboratoryComplejo Hospitalario Universitario de VigoPontevedraSpain
  3. 3.Research LaboratoryComplejo Hospitalario Universitario de VigoPontevedraSpain
  4. 4.Microbiology LaboratoryComplejo Hospitalario de PontevedraPontevedraSpain

Personalised recommendations