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Rapid detection of Staphylococcus aureus bacteremia and methicillin resistance by real-time PCR in whole blood samples

  • N. Wellinghausen
  • D. Siegel
  • S. Gebert
  • J. Winter
Brief Report

Abstract

We prospectively evaluated a real-time polymerase chain reaction (PCR) approach for the rapid diagnosis of Staphylococcus aureus bacteremia and presence of the mecA gene in 902 blood samples from 468 infectious episodes of 384 patients. Eight of 12 blood culture (BC)-confirmed samples were positive by the S. aureus-specific PCR. In addition, the mecA gene PCR correctly detected all cases of BC-confirmed methicillin-resistant Staphylococcus aureus (MRSA) infection. A positive PCR result was also obtained in ten of 462 BC-negative infectious episodes, including three patients with culture-confirmed S. aureus infection at other body sites.

Keywords

Polymerase Chain Reaction Polymerase Chain Reaction Assay Polymerase Chain Reaction Result Methicillin Resistance mecA Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgment

We thank Martin Bommer, Donald Bunjes, Hartmut Döhner, Manfred Hönig, Peter Kern, Wolfgang Lindner, Nikolaus Marx, Ansgar Schulz, Stephan Stilgenbauer, and Heidemarie Suger-Wiedeck for their excellent cooperation regarding the clinical implementation of the study.

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • N. Wellinghausen
    • 1
    • 2
  • D. Siegel
    • 1
  • S. Gebert
    • 1
  • J. Winter
    • 1
  1. 1.Institute of Medical Microbiology and HygieneUniversity Hospital of UlmUlmGermany
  2. 2.Dr. Gaertner & Partner LaboratoriesRavensburgGermany

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